… cleavage reaction on the plasmid then pilot the single strand of … Thus, assembly of the
relaxosome at oriT downregulates … , in the R388 and F-like plasmid systems; IHF binding to oriT of …

… We propose that PcfF initiates relaxosome assembly by … I, and 0.5 mM proteinase inhibitor
AEBSF) before disrupting them … cleavage of the protein as confirmed by SDS-PAGE analysis. …

… , but additionally the relaxosome, a complex of proteins … derivative of plasmid R1-16, which
assembled conjugation … see that the absence of Par proteins decreases in vivo cleavage …

… They are proteins involved in very specific cleavage and strand transfer reactions. Thus …
proteins and IHF bound around the oriT assemble the nucleoprotein complex called relaxosome …

… We also observed that both genomic-plasmid DNA junctions did not occur at the same position
in the … IHF protein inhibits cleavage but not assembly of plasmid R388 relaxosomes. Mol …

… cleavage reaction at the nic site and acts as pilot protein for … Since FinU inhibited both pilus
assembly and entry exclusion, … plasmids was TraG, the T4CP that connects the relaxosome …

… these three proteins, with disulfide bond formation/cleavage likely … assembly of the relaxosome
enabling the rapid spread of … It is presumed that TraY and IHF induce a bend in DNA by 50…

… in a phosphodiesterase cleavage reaction that covalently tethers the … pED208 relaxosome
assembly and engagement with the TraD … Our findings suggest that pED208 relaxosome-TraD …

… self-transmissible plasmids that can mediate their own … used previously, but for the presence
of a photo-activable cleavage site … for the assembly of other components of the relaxosome ( …

… assembly and activity of a protein complex, the relaxosome, … (IHF) and the plasmid-encoded
TraY and TraM proteins, … filament, which stimulates the autocatalytic cleavage of LexA, the …