The extracellular C-loop domain plays an important role in the cell adhesion function of aquaporin 0

Y Nakazawa, M Oka, M Funakoshi-Tago… - Current Eye …, 2017 - Taylor & Francis
… Conclusions: AQP0 is known to bind lipids in the opposing membrane. Our data suggest
that this cell-to-cell adhesion occurs not only in the AQP0/liquids but also via AQP0/AQP0

Molecular mechanism of Aquaporin 0-induced fiber cell to fiber cell adhesion in the eye lens

K Varadaraj, SS Kumari - Biochemical and biophysical research …, 2018 - Elsevier
Intact and C-terminally cleaved AQP0 perform cell-to-cellCell-to-cell adhesion (CTCA),
which is key for establishing lens … AQP0-induced CTCA in intact AQP0 and C-terminally cleaved …

[HTML][HTML] Beyond the channels: adhesion functions of aquaporin 0 and connexin 50 in lens development

Z Li, Y Quan, S Gu, JX Jiang - Frontiers in Cell and Developmental …, 2022 - frontiersin.org
… It is postulated that the cell-to-cell adhesion function of AQP0 serves to increase the proximity
of adjacent fiber cells, thus enhancing the probability of gap junction formation. In primary …

A predominant form of C-terminally end-cleaved AQP0 functions as an open water channel and an adhesion protein in AQP0ΔC/ΔC mouse lens

SS Kumari, K Varadaraj - Biochemical and biophysical research …, 2019 - Elsevier
… a cell-to-cell adhesion (CTCA) protein in a knockin (KI) mouse model (AQP0 ΔC/ΔC ) that
does not express intact AQP0. … of intact and cleaved AQP0 ie, whether both types can perform

Differences in a single extracellular residue underlie adhesive functions of two zebrafish Aqp0s

I Vorontsova, JE Hall, TF Schilling, N Nagai… - Cells, 2021 - mdpi.com
… for adhesion in mammalian AQP0, highlighting the importance of residue 110 in AQP0
cell-to-cell adhesion in vertebrate lenses as well as the divergence of adhesive and water …

Auto-adhesion potential of extraocular Aqp0 during teleost development

F Chauvigné, PG Fjelldal, J Cerdà, RN Finn - PLoS One, 2016 - journals.plos.org
intact AQP0 can perform cell-to-cell adhesion [17]. An alternative suggestion for the low
permeation property of mammalian AQP0 … the Aqp0 channels, we tested the cell-to-cell adhesion

… amino acid residues in the extracellular loops A and C of lens aquaporin 0 interact with the negative charges in the plasma membrane to facilitate cell-to-cell adhesion

S Kumari, G Taginik, S Varadaraj, K Varadaraj - Experimental eye research, 2019 - Elsevier
… (AQP0) are involved in lens fiber cell-to-cell adhesion (CTCA), and the possible mechanism
of CTCA. AQP0 … we used intact and C-terminal end cleaved AQP0 to study the mechanism of …

Experimental and simulation studies of aquaporin 0 water permeability and regulation

JE Hall, JA Freites, DJ Tobias - Chemical reviews, 2019 - ACS Publications
… in zebrafish confirmed that intact functional AQP0 is essential for … between the water channel
and adhesive functions of AQP0. … by performing a simulation of AQP0 with H40 protonated. …

Connexin 50 functions as an adhesive molecule and promotes lens cell differentiation

Z Hu, W Shi, MA Riquelme, Q Shi, S Biswas, WK Lo… - Scientific reports, 2017 - nature.com
adhesion function of AQP0, we conducted a cell adhesion … , we also performed the cell-to-cell
adhesion assay through the … cortical fiber cells exhibit the intact cell membranes (arrows) in …

Connexin 50 and AQP0 are essential in maintaining organization and integrity of lens fibers

S Gu, S Biswas, L Rodriguez, Z Li, Y Li… - … & Visual Science, 2019 - iovs.arvojournals.org
… Besides functioning as a water channel, AQP0 plays a crucial structural role as an adhesion
… to isolate fully intact, which was different from WT and Cx50 and AQP0 single KO lenses. …