[HTML][HTML] Isolation and proteomics of the insulin secretory granule

N Norris, B Yau, MA Kebede - Metabolites, 2021 - mdpi.com
… This review summarizes the proteins identified in the current ISG proteomes from rat
insulinoma INS-1 and INS-1E cell lines. Here, we also discuss techniques of ISG isolation and …

[HTML][HTML] The cells of the islets of Langerhans

G Da Silva Xavier - Journal of clinical medicine, 2018 - mdpi.com
… particular population of islet cells in favour of β-cells? These are all questions to which we
will need answers for islet replacement therapy by transdifferentiation of non-β islet cells to be …

[HTML][HTML] An optimized protocol for purification of functional islets of Langerhans

Y Saliba, JJ Bakhos, T Itani, N Farès - Laboratory Investigation, 2017 - nature.com
Islets of Langerhans and β-cell isolation constitute routinely used … of freshly isolated rat
pancreatic islets stained with dithizone. (e) … similar phenotypic and functional characteristics. …

Establishment of a protocol for the isolation of feline pancreatic islets of Langerhans

I Quiterio Serras Rito Brandao - 2016 - zora.uzh.ch
… specific characteristics. … In rats, yields are of approximately of 600 to 800 islets per animal.
In humans, although the pancreas is thought to contain over one million islets, islet isolation

[HTML][HTML] Comparative study of the effects of confounding factors on improving rat pancreatic islet isolation yield and quality

M Moazenchi, ASH Nejad, M Izadi, M Khalaj… - Cell Journal …, 2022 - ncbi.nlm.nih.gov
… the isolated islet’s properties. We evaluated the variables affecting the rat islet isolation
procedure to reach the maximum islet … be critical for further studies on islet regenerative biology. …

Neurotransmitters and neuropeptides: new players in the control of islet of Langerhans' cell mass and function

ES Di Cairano, S Moretti, P Marciani… - Journal of cellular …, 2016 - Wiley Online Library
Islets cells share many physiological features with neurons, … circuit that regulates insulin
secretion in the islet (Shankar et … insulin secretion in both β-cell lines and isolated islets from …

Characterization of insulin crystalline form in isolated β-cell secretory granules

S Asai, J Moravcová, L Žáková… - Open …, 2022 - royalsocietypublishing.org
… the isolated secretory granules from rat pancreatic Islets of Langerhans and from rat INS-1E …
and MIN6 cells and in rat Langerhans Islets, and the insulin secretory properties of INS-1E …

[HTML][HTML] Insulin release from isolated cat islets of Langerhans

EM Strage, C Ley, GT Westermark… - Domestic Animal …, 2024 - Elsevier
secretion from isolated islets ex vivo. The aim of this study was to establish a protocol for
isolation of islets of Langerhans … disease, and to evaluate insulin secretion responses to various …

[HTML][HTML] Intercellular communication in the islet of langerhans in health and disease

XW Ng, YH Chung, DW Piston - Comprehensive Physiology, 2021 - ncbi.nlm.nih.gov
secretory responses to glucose in intact islets and isolated … In mice and rats, the β-cells make
up 65 to 75% of the islet, … In this section, we will describe some central signaling features of …

[HTML][HTML] Inside the insulin secretory granule

M Germanos, A Gao, M Taper, B Yau, MA Kebede - Metabolites, 2021 - mdpi.com
… In vitro assays using enzymes isolated from rat islets have shown that PC1/3 requires … ,
which although share features in common with the β-cell, have secretory pathways adapted to the …