The Nanoliter Osmometer: Thermal Hysteresis Measurement

N Pariente, M Bar Dolev, I Braslavsky - Ice Binding Proteins: Methods and …, 2023 - Springer
… The nanoliter osmometer is one of the most common tools in the study of ice-binding proteins
… of IBPs but also to explore ice shaping, ice adhesion, and ice growth and melting rates and …

Characterization of ice-binding proteins from sea-ice microalgae

M Bayer-Giraldi, ES Jin, PW Wilson - Plant Cold Acclimation: Methods and …, 2020 - Springer
… Clifton nanoliter osmometer (Clifton Technical Physics, Hartford, NY), but … osmometers
follow the same principle (Otago Osmometer Ltd, Dunedin, New Zealand, or LabVIEW operated

Droplet freezing assays using a nanoliter osmometer

JC Lee, T Hansen, PL Davies - Cryobiology, 2023 - Elsevier
… TH) activity of ice-binding proteins (IBPs), especially antifreeze proteins (AFPs)…
LabVIEW-operated nanoliter osmometer to measure the activity of several bacterial ice nucleation …

Structural and functional analyses of an ice-binding protein from an Antarctic bacterium

M Mangiagalli - 2019 - boa.unimib.it
investigated in the frame of programs for the study and exploitation of biodiversity and provide
a rich reservoir of novel … a LabVIEW-operated custom-designed nanoliter osmometer as …

[PDF][PDF] Ice-binding proteins that accumulate on different ice crystal planes produce distinct thermal hysteresis dynamics

Y Celik, I Braslavsky - Citeseer
… AFPs using a custom-made nanolitre osmometer and a novel microfluidics system. We found
that … onto an ice surface and the measured TH will be investigated further in future studies. …

Falling water ice affinity purification of ice-binding proteins

C Adar, V Sirotinskaya, M Bar Dolev, T Friehmann… - Scientific reports, 2018 - nature.com
Ice-binding proteins (IBPs) permit their hosts to thrive in the presence of … LabVIEW-operated
novel nanoliter osmometer for ice binding protein investigations. J Vis Exp e4189 https://doi.…

Ice-binding proteins and bioinspired synthetic mimics in non-physiological environments

EA Delesky, WV Srubar - Iscience, 2022 - cell.com
… Nature has evolved an effective strategy to mitigate the potentially detrimental effects of ice
formation within organisms. Ice-binding proteins (IBPs) are a robust series of proteins found …

Saturn-shaped ice burst pattern and fast basal binding of an ice-binding protein from an Antarctic bacterial consortium

A Kaleda, L Haleva, G Sarusi, T Pinsky, M Mangiagalli… - Langmuir, 2018 - ACS Publications
… To further investigate ice crystal plane affinity of this protein, we … (27) This high index near-basal
affinity spots are a novel … have been observed in the nanoliter osmometer (Figure 5A–F). …

Measuring antifreeze protein activity

JL Buch - Antifreeze Proteins Volume 2: Biochemistry, Molecular …, 2020 - Springer
… of the newest and most novel approaches within the field. … A good example of this type of
work is the LabVIEW-operated … The ice etching method was first used to investigate binding

Falling Water Ice Purification of Ice-Binding Proteins

C Adar, V Sirotinskaya, MB Dolev, T Friehmann… - 2018 - preprints.org
… 57 In this study, we present a novel ice affinity purification method using a commercial clear
… using a computer-controlled nanoliter 349 osmometer as described elsewhere [36]. 350 …