[PDF][PDF] Responsive antibody conjugates enable quantitative determination of intracellular bond degradation rate
… probes in the cell than internalized probes. Furthermore, the … surface-bound probe-receptor
complexes after the 2-h time … No differences in fluorescence intensity were seen after DTT …
complexes after the 2-h time … No differences in fluorescence intensity were seen after DTT …
Evaluation of an 131I-labeled HER2-specific single domain antibody fragment for the radiopharmaceutical therapy of HER2-expressing cancers
Y Feng, R Meshaw, D McDougald, Z Zhou, XG Zhao… - Scientific Reports, 2022 - nature.com
… of the rationale for developing radiolabeled sdAbs for RPT … the ratio of internalized fraction
over surface bound fraction, … which has incorporated several distinguishing design features: a …
over surface bound fraction, … which has incorporated several distinguishing design features: a …
Chemical analysis of the rituximab radioimmunoconjugates in lyophilized formulations intended for oncological applications
… plasma stability, cellular uptake, internalization, and blocking studies on MCF-7 cells in order
to rapidly identify the promising … (A) Cell uptake calculated as cell surface-bound (•) and …
to rapidly identify the promising … (A) Cell uptake calculated as cell surface-bound (•) and …
Developments and ongoing challenges for analysis of surface-bound proteins
T Weidner, DG Castner - Annual Review of Analytical Chemistry, 2021 - annualreviews.org
… This article describes radiolabeling, surface plasmon resonance, … Technical improvements
(near-total internal reflection … ability to sequence and identify large surface-bound proteins. …
(near-total internal reflection … ability to sequence and identify large surface-bound proteins. …
Preparation and evaluation of radiolabeled antibody recruiting small molecules that target prostate-specific membrane antigen for combined radiotherapy and …
AR Genady, N Janzen, L Banevicius… - Journal of Medicinal …, 2016 - ACS Publications
… (14-17) In addition, there are significant practical advantages to … The goal was to identify
compounds that had high affinity for … were used to calculate surface-bound and internalized …
compounds that had high affinity for … were used to calculate surface-bound and internalized …
Fluorine-18 labeling of an anti-HER2 VHH using a residualizing prosthetic group via a strain-promoted click reaction: Chemistry and preliminary evaluation
… bearing the radiolabel often wash out of the cells decreasing … C, with surface-bound and
internalized activity determined … -2Rs15d, the differences between the two radioconjugates were …
internalized activity determined … -2Rs15d, the differences between the two radioconjugates were …
The biodistribution of therapeutic proteins: Mechanism, implications for pharmacokinetics, and methods of evaluation
KP Conner, SC Devanaboyina, VA Thomas… - Pharmacology & …, 2020 - Elsevier
… , or help to identify off-target tissues at risk for increased TP … antibody/fragment as an IgG
isotype/class-matched internal … However, for the sake of brevity we will limit our discussion of …
isotype/class-matched internal … However, for the sake of brevity we will limit our discussion of …
Application of quantitative protein mass spectrometric data in the early predictive analysis of membrane-bound target engagement by monoclonal antibodies
A Sepp, M Muliaditan - Mabs, 2024 - Taylor & Francis
… target complexes undergo cellular internalization and degradation … Target receptor (brown
structure) exists in surface-bound and … First of all, despite a 25- to 50-fold difference in receptor …
structure) exists in surface-bound and … First of all, despite a 25- to 50-fold difference in receptor …
[HTML][HTML] Radioiodination of extravesicular surface constituents to study the biocorona, cell trafficking and storage stability of extracellular vesicles
SS Yerneni, T Solomon, J Smith… - Biochimica et Biophysica …, 2022 - Elsevier
… cell surface bound EVs prior to imaging the cells. At 37 C, PKH26-labeled EVs internalized
into cells … methodology remains relevant to evaluate any potential EV biocorona differences. …
into cells … methodology remains relevant to evaluate any potential EV biocorona differences. …
Novel PSCA targeting scFv-fusion proteins for diagnosis and immunotherapy of prostate cancer
C Kessler, A Pardo, MK Tur, S Gattenlöhner… - Journal of cancer …, 2017 - Springer
… markers that can accurately distinguish indolent and aggressive … We analyzed the specific
binding and internalization … Surface bound fusion proteins were detected with His6 mAb …
binding and internalization … Surface bound fusion proteins were detected with His6 mAb …