Given that the C580Y polymorphism in the Plasmodium falciparum propeller domain of the
kelch 13 gene (pfk13) was documented in Guyana, monitoring for mutations associated with
antimalarial resistance was undertaken in neighboring Roraima state in Brazil.
Polymorphisms in the pfmdr1 and pfk13 genes were investigated in 275 P. falciparum
samples. No pfk13 mutations were observed. Triple mutants 184F, 1042D, and 1246Y were
observed in 100% of the samples successfully sequenced for the pfmdr1 gene, with 20.1 …

Background Due to the threat of emerging anti-malarial resistance, the World Health
Organization recommends incorporating surveillance for molecular markers of anti-malarial
resistance into routine therapeutic efficacy studies (TESs). In 2018, a TES of artemether-
lumefantrine (AL) and artesunate-amodiaquine (ASAQ) was conducted in Mozambique, and
the prevalence of polymorphisms in the pfk13, pfcrt, and pfmdr1 genes associated with drug
resistance was investigated. Methods Children aged 6–59 months were enrolled in four …