… to cell cycle maintenance and pluripotency in mouse ESCs (… the cells once they had been
blocked in mitosis by nocodazole… Then, we synchronized Flag-Cdk1-expressing mESCs by …

… cells transfected with KIF15 siRNA were synchronized in prometaphase with 200 nM
nocodazole … germline-competent embryonic stem cells of C57BL/6N mouse strain with high …

… 37% of nocodazole-treated cells in metaphase compared to 6% … In the mouse, the high
apparent cloning efficiency of ES cells … donor cells, we first optimized nocodazole synchronization …

… the chromosomes in the metaphase stage oocyte is easily … donor somatic cells to compounds
(eg, nocodazole) that hamper … Synchronization of the cell cycle between the recipient …

… pro-metaphase 1-cell 15 or interphase 2-cell 16 embryos, … Since ES cells transit through
a quasi-2-cell embryonic state … ) and is no longer synchronised with maternal chromatin …

… , I combined cell cycle synchronisation mediated by nocodazole with our well-… Mouse
embryonic stem cells … Due to their origin, these cells are called embryonic stem cells and provide a …

… nocodazole addition. (C) Immunofluorescence images of spherical condensates in acutely
10 μM nocodazole–treated mouse metaphase I … spindles, cells were synchronized with 10 μM …

… modelling or require cell synchronization. We describe here … BrdU antibodies by staining
mouse embryonic fibroblasts (MEF… Since these cells are known to be insensitive to nocodazole […

… We further showed that ES cells with high levels of OCT4 in … , ES cells were early on used
to generate knock-out mice by … (A) Fluorescence snapshots of living ES cells in metaphase …

… , cell cycle synchronization of donor cells, enucleation, cell … the metaphase stage can result
in successful cloning using … in many studies that use ES cells or oocytes as a reprogramming …