Practical Guidance for Developing Small-Molecule Optical Probes for In Vivo Imaging

C Ran, JR Mansfield, M Bai, NT Viola… - Molecular Imaging and …, 2023 - Springer
… The focus of this paper is preclinical animal studies with small… ,23,24], and fluorescent protein
engineering is a highly active … the emission after normalizing with imaging acquisition time, …

Early detection of acute drug-induced liver injury in mice by noninvasive near-infrared fluorescence imaging

KO Vasquez, JD Peterson - Journal of Pharmacology and Experimental …, 2017 - ASPET
… multiple animal imaging and sensitive detection of the liver signal. The 2D transillumination
… AS-680 to allow for subtraction or normalization to compensate for vascular effects. In our …

Endogenous fluorescent proteins in the mucus of an intertidal Polychaeta: clues for biotechnology

AP Rodrigo, A Lopes, R Pereira, SI Anjo, B Manadas… - Marine Drugs, 2022 - mdpi.com
… –green fluorescence when subjected to UV transillumination (… % among replicates normalized
to 1 mg total protein, mL −1 . … on fluorochromes from marine animals. As an example, the …

Fluorescence‐and multispectral optoacoustic imaging for an optimized detection of deeply located tumors in an orthotopic mouse model of pancreatic carcinoma

J Napp, MA Stammes, J Claussen… - … journal of cancer, 2018 - Wiley Online Library
… ) with the planar fluorescence imaging systems Optix MX2 (… ; epi-/trans-illumination system)
as well as the small animalFluorescence intensities are displayed in normalized counts. …

In Vivo Flow Cytometry of Extremely Rare Circulating Cells

X Tan, R Patil, P Bartosik, JM Runnels, CP Lin… - Scientific reports, 2019 - nature.com
fluorescence microscopes that operate in trans-illumination … In combination with fluorescent
dyes, fluorescent proteins and … We also normalized the amplitude between channels 1 and 2…

Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts

RW Bourdeau, A Lee-Gosselin, A Lakshmanan… - Nature, 2018 - nature.com
… d, Normalized ultrasound contrast as a function of IPTG … using spectrally distinct fluorescent
proteins. Analogous acoustic … blue-light transillumination, resulting in mWasabi fluorescence. …

New tools for redox biology: from imaging to manipulation

DS Bilan, VV Belousov - Free Radical Biology and Medicine, 2017 - Elsevier
… to animal neurons to … , a fluorescent protein is integrated via short peptide linkers into the
most conformationally mobile region of the regulatory OxyR domain. Thus, a fluorescent protein

Signal and measurement considerations for human translation of diffuse in vivo flow cytometry

F Ivich, J Pace, AL Williams, M Shumel… - Journal of …, 2022 - spiedigitallibrary.org
… -illumination (as opposed to trans-illumination) geometry, in … Figure 4 shows the normalized
MC sensitivity calculations … pronounced in our Green Fluorescent Protein (GFP) compatible …

Visualizing Phagocytic Cargo In Vivo from Engulfment to Resolution in Caenorhabditis elegans

G Fazeli, J Frondoni, S Kolli, AM Wehman - … and Phagosomes: Methods …, 2023 - Springer
… clearance and methods to normalize this process to distinguish … embryos, ideally with oblique
transillumination using a mirror. … by tagging a protein of interest with a fluorescent protein. …

N‐Cadherin, a novel and rapidly remodelling site involved in vasoregulation of small cerebral arteries

Z Sun, M Li, Z Li, MA Hill… - The Journal of …, 2017 - Wiley Online Library
… process of enhanced green fluorescent protein (EGFP)-… Right: overlay of the fluorescence
image and trans-illumination … PE treatment, fluorescence signal was normalized by the …