Permeabilization of Escherichia coli with ampicillin for a whole cell biocatalyst with enhanced glutamate decarboxylase activity

W Zhao, S Hu, J Huang, P Ke, S Yao, Y Lei… - Chinese Journal of …, 2016 - Elsevier
… beads generated in the present study was investigated in terms of its reusability in the
biotransformation of L-MSG to GABA at 37 C (Fig. 4). The results showed that the GABA yield with …

[HTML][HTML] Effect of cell permeability and dehydrogenase expression on octane activation by CYP153A6-based whole cell Escherichia coli catalysts

BE White, CJ Fenner, MS Smit, STL Harrison - Microbial Cell Factories, 2017 - Springer
… This study focused on the biotransformation of n-octane to 1-octanol using resting
Escherichia coli cells expressing the CYP153A6 operon, which includes the electron transport …

[HTML][HTML] Biosynthesis of S-Adenosylmethionine by Magnetically Immobilized Escherichia coli Cells Highly Expressing a Methionine Adenosyltransferase Variant

C Yin, T Zheng, X Chang - Molecules, 2017 - mdpi.com
… The use of whole-cells for biosynthesis and biotransformation … were used to permeabilize
the free whole-cells of E. … trimethyl ammonium bromide) significantly increased the cell-bound …

An efficient method for the release of recombinant penicillin g amidase from the Escherichia coli periplasm

SM Lee - 생명과학회지, 2017 - dbpia.co.kr
… PGA may be useful in a process of biotransformation for other industrial purposes. …
Periplasmic penicillin G acylase activity in recombinant Escherichia coli cells permeabilized

… Biotransformation of Ethyl 4-Chloro-3-Oxobutanoate into Ethyl (S)-4-Chloro-3-Hydroxybutanoate by Recombinant E. coli CCZU-T15 Whole Cells in [ChCl][Gly] …

Y Dai, B Huan, HS Zhang, YC He - Applied biochemistry and …, 2017 - Springer
coli CCZU-T15, respectively. TEM image indicated that the cell membrane was permeabilized
and lost its integrity and when the cell was exposed to [ChCl][Gly]–water media with Tween…

Quaternary ammonium surfactant structure determines selective toxicity towards bacteria: mechanisms of action and clinical implications in antibacterial prophylaxis

ÂS Inácio, NS Domingues, A Nunes… - Journal of …, 2016 - academic.oup.com
cell viability, membrane integrity, cell cycle and metabolism … The percentage of permeabilized
propidium iodide + cells … The n-alkyl chain length of the trimethylammonium bromide (TAB) …

… bottlenecks: expression of complementary enzymes and permeabilization of cell membranes to improve performance of CYP153A6 in Escherichia coli whole cell …

BE White - 2022 - open.uct.ac.za
… sites”; lack of oxygen for biotransformation and cell metabolism; poor transport of n-octane …
biotransformation rates or product titres in biotransformations carried out with whole cells. …

[HTML][HTML] Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical …

Y Wang, S Sun, L Yu, S Hu, W Fan, F Leng… - Ultrasonics Sonochemistry, 2021 - Elsevier
… The cetyl trimethyl ammonium bromide (CTAB) was also … , which depends on the growth
and metabolism process of E. … of temperature on the outer membrane permeabilization of E. …

[PDF][PDF] Chowdary, with permeabilised cells of

K Deepthi Priya, MV Petkar - Gluconobacter japonicus - researchgate.net
… aspect of permeabilization is the production of cells suitable to use biotransformation system…
solution prepared with cetyl trimethyl ammonium bromide of 0.2% (w/v) in 0.1M citrate buffer …

[HTML][HTML] L-Carnitine Production Through Biosensor-Guided Construction of the Neurospora crassa Biosynthesis Pathway in Escherichia coli

P Kugler, M Trumm, M Frese… - … in Bioengineering and …, 2021 - frontiersin.org
… of L-carnitine out of the E. coli cell may also limit biotransformation and its de novo production.
… exchange of intracellular and extracellular trimethylammonium compounds such as L-…