[HTML][HTML] Hydrogen Sulfide Sensitizes Acinetobacter baumannii to Killing by Antibiotics

SY Ng, KX Ong, ST Surendran, A Sinha… - Frontiers in …, 2020 - frontiersin.org
… over 98% of membrane permeabilization. Samples were processed … These peptides were
reconstituted in triethylammonium … Energy metabolism was affected as indicated by a reduced …

Real-time monitoring of NADPH levels in living mammalian cells using fluorescence-enhancing protein bound to NADPHs

A Roshanzadeh, H Kang, SH You, J Park… - Biosensors and …, 2019 - Elsevier
… , it required protein engineering or membrane-permeabilizing … the detection of NADPH
metabolism in mammalian cells, we … bacterial cells after the treatment of cetyl trimethylammonium

[HTML][HTML] The two weapons against bacterial biofilms: Detection and treatment

A Cruz, M Condinho, B Carvalho, CM Arraiano… - Antibiotics, 2021 - mdpi.com
… The carbohydrate transport and metabolism has been reported as an essential tool for …
coli and S. aureus, L-PEI was shown to be able to permeabilize and disrupt bacterial cell

Vancomycin C-terminus guanidine modifications and further insights into an added mechanism of action imparted by a peripheral structural modification

ZC Wu, MD Cameron, DL Boger - ACS infectious diseases, 2020 - ACS Publications
trimethylammonium cation (C1) modification on vancomycin induces bacteria cell
permeabilization … modification are not subject to rapid metabolism. These preliminary studies …

Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function

W Jang, D Puchkov, P Samsó, YT Liang… - Science, 2022 - science.org
… pathway to control oxidative cell metabolism uncovered in this … For LC3-II staining, cells were
permeabilized with 20 μM … same buffer) recombinant Escherichia coli BL21 expressed GST-…

[PDF][PDF] High glucose triggers nucleotide imbalance through O-GlcNAcylation of key enzymes and induces KRAS mutation in pancreatic cells

CM Hu, SC Tien, PK Hsieh, YM Jeng, MC Chang… - Cell metabolism, 2019 - cell.com
… and sugar metabolism exists. Here, we show that under high-glucose conditions, cellular
O-… Flow Cytometry for KRASG12D Analysis The cells were fixed and permeabilized with ice-…

Production, purification, immobilization and application of penicillin acylase from Acremonium sclerotigenum

J Lata - 2020 - repository.tribal.gov.in
Bioconversion of penicillin G to 6 aminopenicillanic acid at … , USA, fermenter used for the
bioconversion of penicillin G to 6-APA … subtilis was cloned and expressed in Escherichia coli, …

Type III secretion system effectors form robust and flexible intracellular virulence networks

…, J Sanchez-Garrido, Z Kozik, E Nunez-Berrueco… - Science, 2021 - science.org
… pathotype (eg, clinical Escherichia coli isolates encode between 21 … and DNA replication
(E) and cholesterol metabolism (F). … in PBS for 20 min at RT and permeabilized in 0.1% (v/v) of …

[HTML][HTML] Effect of a Novel Alpha/Beta Hydrolase Domain Protein on Tolerance of K. marxianus to Lignocellulosic Biomass Derived Inhibitors

D Wu, D Wang, J Hong - Frontiers in Bioengineering and …, 2020 - frontiersin.org
… , the permeabilized plasma membrane of a dead cell will … O 2 and 2-mercaptoethyl-trimethylammonium
iodide acetate. Thus, … to inhibitors, cells regulated their energy metabolism toward …

Comparative target analysis of chlorinated biphenyl antimicrobials highlights MenG as a molecular target of triclocarban

R Macsics, MW Hackl, C Fetzer, D Mostert… - Applied and …, 2020 - Am Soc Microbiol
… aureus menaquinone metabolism via inhibition of the … only accessible if the cell membrane
is permeabilized. As a positive … Following dilution with 50 mM triethylammonium bicarbonate …