… component of tissues with high physiological relevance. It takes … compounds in Escherichia
coli. Trimethylammonium … was developed and genetic engineering strategies were identified …

… 1 mM IPTG and the culture was continued up to an OD 600 of ∼… While interesting from a
physiological point of view, it was … plasmid pTrc99A-TML2Car revealed L-carnitine production …

… constant supply of L-carnitine through different metabolic networks. The upregulation of
numerous enzymes including L-carnitine … in 1905 Its major physiological function, its biosynthetic …

… plasmid was used to transform the Ylcrc1 knock-out strain. … to transport, propionyl-L-carnitine
in exchange with L-carnitine [11]. … Escherichia coli DH5α strains were grown at 37 C in LB …

… CFE to produce l-carnitine when incubated with lysine and acetate would suggest an
important contribution of this pathway in the enhanced production of l-carnitine observed under …

… the nonevolved parental strain enabled l-carnitine-dependent … After transforming the resulting
plasmids to Escherichia coli … Unless indicated otherwise, genetic engineering was done …

… plasmid stability in three industrially relevant Escherichia coli … retention in comparison to
free cells and boosting l-carnitine … to be a global physiological phenomenon occurring in …

… pathways in Escherichia coli to selectively produce three … or design principles for genetic
engineering of non-oleaginous … in the parent strain showed Lcarnitine-dependent growth on …

… heterologously expressed in Escherichia coli and the corresponding … The physiological
electron acceptor of BcoC remains to be … The sequence of the resulting plasmids was verified. …