The European antibody network's practical guide to finding and validating suitable antibodies for research

G Roncador, P Engel, L Maestre, AP Anderson… - MAbs, 2016 - Taylor & Francis
antibody validation because of the adverse effect that poor … of antibodies to a single protein,
eg, c-FLIPCitation 12 or to … many researchers through the maze of antibody validation and …

[HTML][HTML] Although it's painful: The importance of stringent antibody validation

VA Meliopoulos, S Schultz-Cherry - PLoS Pathogens, 2018 - journals.plos.org
… with low-abundance proteins, for which the antibody in question can only detect high levels
… We would not have known there was a problem with antibody 1 if we had not used KO mice …

Antibody validation in bioimaging applications based on endogenous expression of tagged proteins

M Skogs, C Stadler, R Schutten… - … of proteome research, 2017 - ACS Publications
… Some researchers suggest that the problem is best tackled by only using recombinant …
for the target protein as well as an anti-GFP antibody in order to detect even low-abundance …

Current challenges of metastatic breast cancer

B Lim, GN Hortobagyi - Cancer and Metastasis Reviews, 2016 - Springer
… early detection and improvement in survival owing to advancements in biomedical research
… in stage IV breast cancer, and we finish by noting new research initiatives to improve poor

[HTML][HTML] The pyrrolo-1, 5-benzoxazepine, PBOX-15, enhances TRAIL‑induced apoptosis by upregulation of DR5 and downregulation of core cell survival proteins in …

SM Nathwani, LM Greene, S Butini… - International …, 2016 - spandidos-publications.com
… Apoptotic defects are frequently associated with poor outcome … Cell surface expression of
the receptors was then detected by … PBOX-15 can downregulate c-FLIP, resulting in increased …

[HTML][HTML] A microplate-based nonradioactive protein synthesis assay: application to TRAIL sensitization by protein synthesis inhibitors

CJ Henrich - PLoS One, 2016 - journals.plos.org
detection using anti-puromycin antibodies have been previously reported and well-validated. …
However, the low throughput nature of the applications described to date (cellular imaging, …

Nanoparticles-based drug delivery and gene therapy for breast cancer: Recent advancements and future challenges

Z Mirza, S Karim - Seminars in cancer biology, 2021 - Elsevier
… and suffer concerns of low bioavailability, low cellular uptake, … antibodies allows both in vitro
and ex vivo optical diagnosis, … by activating caspase-8 and c-FLIP, but due to the changes in …

[HTML][HTML] Bortezomib in Combination with Physachenolide C Reduces the Tumorigenic Properties of KRASmut/P53mut Lung Cancer Cells by Inhibiting c-FLIP

T Kanagasabai, Z Dunbar, SG Ochoa, T Farris… - Cancers, 2024 - mdpi.com
… , and the maximal c-FLIP expression was detected in castrate-… Cancer cells are often exposed
to a metabolically challengingstudy was to maximize the anticancer potential by using low

Anti-GPC3 antibody-modified sorafenib-loaded nanoparticles significantly inhibited HepG2 hepatocellular carcinoma

X Tang, L Chen, A Li, S Cai, Y Zhang, X Liu, Z Jiang… - Drug …, 2018 - Taylor & Francis
… To overcome this problem, we developed a novel SFB-loaded … Primary antibodies for Bad
and XIAP were purchased from … apoptosis and downregulates Mcl-1 and c-FLIP expression. …

Generation and validation of monoclonal antibodies against the maltose binding protein

YN Park, RA Glover, KJ Daniels… - Monoclonal Antibodies in …, 2016 - liebertpub.com
… MBP precipitates on the western blot were detected with rabbit polyclonal anti-MBP
antibody (Thermo Fisher Scientific). The Odyssey dye IR Dye 800-conjugated goat anti-rabbit …