… in enzyme engineering, it has become possible to improve catalytic … (h) Inverting GPs promote
synthesis via either axial-to-… Most glycosidases used for synthetic purposes have an exo-…

… from rationally modifying specific catalytic residues, over targeted … to promote different types
of HMO synthesis reactions. The … were obtained with engineered glycosidases compared to …

… engineered glycosidases or glycosyl hydrolases (GHs) offer a promising avenue for the
synthesis of glycans like oligosaccharides … results of federally sponsored research in accordance …

… to their prebiotic action and other health-promoting effects, in addition to other properties, …
glycosidases with transferase capacity or glycosyltransferases. In both cases, the catalytic …

… makes glycosidases and phosphorylases applicable to the … The active site of ENGases
contains two catalytic residues: a … (75i) With the emergence of new tools of genetic engineering, …

… of either the catalytic base residue of inverting glycosidases or the nucleophile of retaining
… the protein engineering methods and reaction condition control to promote the fucosidase-…

… CAZyme substrate and catalytic promiscuity by reactions as … was recently engineered to
produce fructo-oligosaccharides from sucrose … in combination with strain-promoted azide-alkyne …

… control strategy and together with protein engineering. … and displacing the hydration layer in
the catalytic site of the enzyme [… thermophilus β-glycosidase variants with reduced hydrolytic …

… and a non-catalytic loop domain that promoted acceptor sugar … engineered to glycosynthases
by mutating their catalytic … in glycosyl transferase and modified glycosidase reactions. The …

… advances in synthetic biology have made the engineering of … Its promising health-promoting
benefits to infants stimulated … transfer activity in a catalytic nucleophile mutant allows to use …