Glycan/Protein-Stable Isotope Labeling in Cell Culture for Enabling Concurrent Quantitative Glycomics/Proteomics/Glycoproteomics
… -based quantitation between different cell lines. Stable isotope labeling by amino acids in a
cell culture (SILAC) is the most widely used metabolic labeling method in proteomic analysis. …
cell culture (SILAC) is the most widely used metabolic labeling method in proteomic analysis. …
Advances in stable isotope labeling: dynamic labeling for spatial and temporal proteomic analysis
NC Beller, AB Hummon - Molecular omics, 2022 - pubs.rsc.org
… The use of isotopically labeled amino acids was developed … Stable Isotope Labeling by
Amino Acids in Cell culture (SILAC… forms of heavy isotopic labels to quantify relative differences …
Amino Acids in Cell culture (SILAC… forms of heavy isotopic labels to quantify relative differences …
Impact of cystinosin glycosylation on protein stability by differential dynamic stable isotope labeling by amino acids in cell culture (SILAC)
N Nevo, L Thomas, C Chhuon, Z Andrzejewska… - … & Cellular Proteomics, 2017 - ASBMB
… stable and undergoes faster degradation. Our dynamic stable isotope labeling by amino acids
in cell culture (… For dynamic SILAC studies, NIH/3T3 cells stably expressing different fusion …
in cell culture (… For dynamic SILAC studies, NIH/3T3 cells stably expressing different fusion …
… -and autophagy-associated molecules in human lung cancer A549 cells in response to cisplatin treatment using stable isotope labeling with amino acids in cell culture
Z Wang, G Liu, J Jiang - International Journal of …, 2019 - spandidos-publications.com
… Stable isotope labeling by amino acids in cell culture (SILAC) is effective in distinguishing
… The incorporation of stable isotopes facilitates the quantitative recognition of the differences …
… The incorporation of stable isotopes facilitates the quantitative recognition of the differences …
Advances and applications of stable isotope labeling-based methods for proteome relative quantitation
… with different stable isotope reagents are combined into the same LC-MS/MS run [15]. … use
in proteome-wide quantitation. Stable isotope labeling by amino acids in cell culture (SILAC) […
in proteome-wide quantitation. Stable isotope labeling by amino acids in cell culture (SILAC) […
Time-resolved analysis of proteome dynamics by tandem mass tags and stable isotope labeling in cell culture (TMT-SILAC) hyperplexing
KA Welle, T Zhang, JR Hryhorenko, S Shen… - … & Cellular Proteomics, 2016 - ASBMB
… different SILAC labeling time-points are reacted with different … At different time-points, a
peptide generated from the same … labeled amino acids are introduced to cultured cells. Over time…
peptide generated from the same … labeled amino acids are introduced to cultured cells. Over time…
Spatial stable isotopic labeling by amino acids in cell culture: Pulse-chase labeling of three-dimensional multicellular spheroids for global proteome analysis
NC Beller, JK Lukowski, KR Ludwig… - Analytical …, 2021 - ACS Publications
… isotopic labels could be incorporated into the proteins of the individual spheroid cellular
layers by using timed cell culture … for the comparison of different isotopic labels among each of …
layers by using timed cell culture … for the comparison of different isotopic labels among each of …
… of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC)
V Llombart, T Garcia-Berrocoso, JJ Bech-Serra… - … of Proteomics, 2016 - Elsevier
… because of different peptide grouping or different protein assignments based on identical …
without any signal peptide identified along its amino acid sequence (Supplementary Table IV). …
without any signal peptide identified along its amino acid sequence (Supplementary Table IV). …
Classification-based quantitative analysis of stable isotope labeling by amino acids in cell culture (SILAC) data
… quantitative proteomics method is stable isotope labeling that either introduces a mass
difference … and powerful approach for stable isotope labeling is stable isotope labeling of amino …
difference … and powerful approach for stable isotope labeling is stable isotope labeling of amino …
Complete Native Stable Isotope Labeling by Amino Acids of Saccharomyces cerevisiae for Global Proteomic Analysis
S Dannenmaier, SB Stiller, M Morgenstern… - Analytical …, 2018 - ACS Publications
… labeling by amino acids in cell culture (SILAC). However, … ) into the proteome of BY4741
grown on different carbon sources. … of the mitochondrial proteome shows that the cytochrome c …
grown on different carbon sources. … of the mitochondrial proteome shows that the cytochrome c …