Glycan/Protein-Stable Isotope Labeling in Cell Culture for Enabling Concurrent Quantitative Glycomics/Proteomics/Glycoproteomics

P Jiang, W Peng, J Zhao, M Goli, Y Huang… - Analytical …, 2023 - ACS Publications
… -based quantitation between different cell lines. Stable isotope labeling by amino acids in a
cell culture (SILAC) is the most widely used metabolic labeling method in proteomic analysis. …

Advances in stable isotope labeling: dynamic labeling for spatial and temporal proteomic analysis

NC Beller, AB Hummon - Molecular omics, 2022 - pubs.rsc.org
… The use of isotopically labeled amino acids was developed … Stable Isotope Labeling by
Amino Acids in Cell culture (SILAC… forms of heavy isotopic labels to quantify relative differences

Impact of cystinosin glycosylation on protein stability by differential dynamic stable isotope labeling by amino acids in cell culture (SILAC)

N Nevo, L Thomas, C Chhuon, Z Andrzejewska… - … & Cellular Proteomics, 2017 - ASBMB
stable and undergoes faster degradation. Our dynamic stable isotope labeling by amino acids
in cell culture (… For dynamic SILAC studies, NIH/3T3 cells stably expressing different fusion …

… -and autophagy-associated molecules in human lung cancer A549 cells in response to cisplatin treatment using stable isotope labeling with amino acids in cell culture

Z Wang, G Liu, J Jiang - International Journal of …, 2019 - spandidos-publications.com
Stable isotope labeling by amino acids in cell culture (SILAC) is effective in distinguishing
… The incorporation of stable isotopes facilitates the quantitative recognition of the differences

Advances and applications of stable isotope labeling-based methods for proteome relative quantitation

J Liu, Y Shan, Y Zhou, Z Liang, L Zhang… - TrAC Trends in Analytical …, 2020 - Elsevier
… with different stable isotope reagents are combined into the same LC-MS/MS run [15]. … use
in proteome-wide quantitation. Stable isotope labeling by amino acids in cell culture (SILAC) […

Time-resolved analysis of proteome dynamics by tandem mass tags and stable isotope labeling in cell culture (TMT-SILAC) hyperplexing

KA Welle, T Zhang, JR Hryhorenko, S Shen… - … & Cellular Proteomics, 2016 - ASBMB
different SILAC labeling time-points are reacted with different … At different time-points, a
peptide generated from the same … labeled amino acids are introduced to cultured cells. Over time…

Spatial stable isotopic labeling by amino acids in cell culture: Pulse-chase labeling of three-dimensional multicellular spheroids for global proteome analysis

NC Beller, JK Lukowski, KR Ludwig… - Analytical …, 2021 - ACS Publications
isotopic labels could be incorporated into the proteins of the individual spheroid cellular
layers by using timed cell culture … for the comparison of different isotopic labels among each of …

… of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC)

V Llombart, T Garcia-Berrocoso, JJ Bech-Serra… - … of Proteomics, 2016 - Elsevier
… because of different peptide grouping or different protein assignments based on identical …
without any signal peptide identified along its amino acid sequence (Supplementary Table IV). …

Classification-based quantitative analysis of stable isotope labeling by amino acids in cell culture (SILAC) data

S Kim, N Carruthers, J Lee, S Chinni… - Computer methods and …, 2016 - Elsevier
… quantitative proteomics method is stable isotope labeling that either introduces a mass
difference … and powerful approach for stable isotope labeling is stable isotope labeling of amino

Complete Native Stable Isotope Labeling by Amino Acids of Saccharomyces cerevisiae for Global Proteomic Analysis

S Dannenmaier, SB Stiller, M Morgenstern… - Analytical …, 2018 - ACS Publications
labeling by amino acids in cell culture (SILAC). However, … ) into the proteome of BY4741
grown on different carbon sources. … of the mitochondrial proteome shows that the cytochrome c …