… extracts was used to compare the overall cell harvest and cell lysis efficiencies among … 0.5
mL of 0.25% trypsin/EDTA (Hyclone, Logan, Utah) was added and incubated with cell cultures …

… Stable isotope labeling by amino acids in cell culture (SILAC) has been widely utilized in
bottom-up proteomics. (… same type of amino acids suffer from different local microenvironments, …

… label-based proteomics and on chemical labeling in particular, we give a brief introduction
of label-free proteomics in comparison… stable isotope labeling by amino acids in cell culture (…

… stable isotope labeled amino acids to label proteins for turnover experiments especially in cell
culture… that vary so that labeling ratios of the labeled atoms in different amino acids are not …

… By using heavy isotope labeling in cell culture combined with … (PTMs), which can bestow
different biophysical properties to … of amino acids or small molecules bearing stable isotopes, …

… cell line can be grown as an adherent monolayer or as 1 mm spheroids. Using a stable isotope
labeling of amino acids (SILAC) labeling … phosphoprotein abundance differences in two …

… of unavailable standards compounds with stable isotope labeling is usually one of the biggest
… Based on different labeling chemistry, a number of stable isotope labeling reagents have …

… -based proteomics can be used to monitor proteome dynamics. Pulse stable isotope labelling
by amino acids in cell culture (… To compare our proteomic result (asynchronous HeLa cells) …

… Stable isotope labeling by amino acids in cell culture (SILAC) has become very popular as a
quantitative proteomic … metabolic labeling strategy in which isotope-labeled amino acids are …

… dual-labeling experiments are straightforward in cell culture, it is … heavy amino acids followed
by in silico analyses of stable isotope … employed five different types of stable isotope labels. …