… reference genes (RGs) for cotton gene expression normalization … processes in cotton, such
as fiber development and biotic and … polymerase chain reaction (qRT-PCR) or quantitative …

… Compared to all textile fibers except cotton, jute is one of the cheapest and most important
… of reference genes to identify the most suitable internal control for normalization of qRT-PCR …

… data normalization, 18s rRNA was used as an internal control… A flower with the good and
healthy condition was selected … The qRT-PCR assay was optimized by running a series of …

… All expression level was normalized by samples. (d) The … , we conducted qRT-PCR analyses
of two selected genes at … -regulated gene pannel suitable for fiber improvement by CRISPR. …

… Through this study, six potential candidate internal control genes from Gossypium arboreum
… Suitable internal control genes for qRTPCR normalization in cotton fiber development and …

… GhUBI1 was used as an internal control for normalization. (B) … of fiber elongation and SCW
synthesis to form the appropriate … of cotton genes was analyzed by qRT-PCR using a cotton …

… genes have been reported to be involved in cotton fiber … The expression levels of miRNA
were normalized by using U6 … technical replicates were used for qRT-PCR analysis. The error …

… ) genes in cotton fiber development need be further explored. … in the overexpression lines
according to qRT-PCR analysis; for … hirsutum samples were normalized by calibrating with the …

… were normalized by the optical examination of an external spot set … ) and 18S RNA were
used as internal controls. The … We used qRT-PCR to validate the expression patterns identified …

… control for normalization. The values are means ± SDs of … These results revealed that
appropriate levels of GhGalT1 … We designed gene-specific primers for each gene and qRT-PCR …