[HTML][HTML] Identification and expression analysis of Tubulin gene family in upland cotton

B Chen, J Zhao, G Fu, X Pei, Z Pan, H Li… - Journal of Cotton …, 2021 - Springer
qRT-PCR analysis revealed that 12 α-tubulin and 7 β-tubulin … of all values, then were
normalized with the log 2 (FPKM+ 1) … GhUBQ (GhA10G005800) gene as an internal control, and a …

[HTML][HTML] Transcriptome analysis and identification of genes associated with fruiting branch internode elongation in upland cotton

F Ju, S Liu, S Zhang, H Ma, J Chen, C Ge, Q Shen… - BMC plant biology, 2019 - Springer
… Our RNA-Seq and qRT-PCR results indicate that the … reads for each gene was then counted
and normalized into reads … for the selected genes and the internal control gene (UBQ) are …

[HTML][HTML] Genome-wide identification and characterization of TALE superfamily genes in cotton reveals their functions in regulating secondary cell wall biosynthesis

Q Ma, N Wang, P Hao, H Sun, C Wang, L Ma, H Wang… - BMC plant biology, 2019 - Springer
… ) method was employed to normalize the total short read … cotton varieties with different fiber
quality by qRT-PCR. a The … large cotton varieties have also been performed, providing a good

[HTML][HTML] The miR319-targeted GhTCP4 promotes the transition from cell elongation to wall thickening in cotton fiber

JF Cao, B Zhao, CC Huang, ZW Chen, T Zhao, HR Liu… - Molecular plant, 2020 - cell.com
Cotton fiber cells are developmentally synchronous, highly elongated, and contain nearly …
role in balancing cotton fiber cell elongation and wall synthesis. During fiber development the …

[HTML][HTML] Selection of Stable Housekeeping Genes for qPCR in Biotic and Abiotic Stressed Jute and their Application in Lignin Pathway Analysis

S Parida, SK Datta, N Dey, S Majumder - Plant Stress, 2024 - Elsevier
gene expression via qRT-PCR, a reliable housekeeping gene (HKG), also known as an internal
control gene … of 2 genes would be sufficient for qRT-PCR data normalisation (Figure 4A). …

The comparative transcriptome and co-expression of hub genes analysis for fiber development in RIL populations of upland cotton

R Wei, Y Zhong, N Xu, P Li, MT Azhar, Z Yang… - Industrial Crops and …, 2024 - Elsevier
… However, fiber quality in upland cotton is not as good as that … The trend of qRT-PCR results
and RNA-seq results were … of the candidate genes determined by qRT-PCR, which was …

Data mining of transcriptional biomarkers at different cotton fiber developmental stages

P Prasad, U Khatoon, RK Verma, SV Sawant… - Functional & Integrative …, 2022 - Springer
… to the good quality and high yield verities of cotton which are … quantitative real-time PCR
(qRT-PCR) analysis in which they were … the normalization process, a total of 31,800 genes were …

[HTML][HTML] Genome-wide analysis of cotton GH3 subfamily II reveals functional divergence in fiber development, hormone response and plant architecture

D Yu, G Qanmber, L Lu, L Wang, J Li, Z Yang, Z Liu… - BMC plant biology, 2018 - Springer
… In addition, reconstruction of ideal plant architecture is … GhHis 3 was used as an internal
control to normalize the … expression changes of GhGH3 genes by qRT-PCR at five different time …

GhbHLH18 negatively regulates fiber strength and length by enhancing lignin biosynthesis in cotton fibers

Z Gao, W Sun, J Wang, C Zhao, K Zuo - Plant Science, 2019 - Elsevier
… (A) qRT-PCR analysis indicates that GhbHLH18 gene expression is repressed in cotton
fibers of … The expression levels of GhbHLH18 were normalized to those of the internal control

[HTML][HTML] Overexpression of a sucrose synthase gene indirectly improves cotton fiber quality through sucrose cleavage

M Ahmed, A Iqbal, A Latif, SU Din, MB Sarwar… - Frontiers in Plant …, 2020 - frontiersin.org
… SuS expression levels were normalized with GAPDH. For the … using qRT-PCR with 2 μl of
cDNA from the four transgenic cottoncotton produced in this study is the best source for use in …