… first set of suitable reference genes for qRT-PCR analyses, … one reference gene as internal
controls. The primers used for … genes that can be employed for qRT-PCR normalization. …

… selected GhNAC genes in fiber development by qRT-PCR. … was used as the internal control
to calculate and normalize the … create new cotton varieties possessing good agronomic traits …

… most suitable candidate reference genes for normalisation of … , the tubes were covered with
cotton along 1/4 tube length … EF1-α was the best optimal internal control genes in almost all of …

… transcriptome data was verified by qRT-PCR assay. Total 62 … quality during cotton fiber
development, we speculate that the ratio … ) gene was used as an internal control to normalize the …

… The relative transcript level was determined and normalized … best, followed by Sea island
cotton Pima 3–79 and Upland … in developing fibers of SY6167 and TM-1 using qRT-PCR. A …

… The data were normalized, and FPKM (Fragments per … From the results of qRT-PCR, we
can observe that the expression … GhUBQ7 (DQ116441) was used as the internal control. Each …

… during the fiber development stage, and the qRT-PCR analysis … as an internal reference gene
to normalize the cDNA … in cotton is a highly polygenic trait and is suitable for QTL analyses. …

… to 0.9, we finally obtain an appropriate soft-threshold value of 14, … levels determined by
qRT-PCR were normalized to the … effectors, reporter and internal control vectors used in transient …

… gene AtACTIN in Arabidopsis was used as an internal control… mapped (FPKM) normalized
log10 (transformed counts). The … We then performed qRT-PCR of some genes in the …

… Cotton actin2, an internal control for constitutive expression, … of its target gene was performed
also using qRT-PCR. The … The unique reads were normalized to give reads per million and …