Screening of abiotic stress‐responsive cotton genes using a cotton full‐length cDNA overexpressing Arabidopsis library
S Li, H Chen, Z Hou, Y Li, C Yang… - Journal of Integrative …, 2020 - Wiley Online Library
… Because various abiotic and biotic stresses strongly influence cotton fiber yield and quality,
… qRT-PCR) and normalized by the 2 −ΔΔCt method using the UBQ gene as an internal control…
… qRT-PCR) and normalized by the 2 −ΔΔCt method using the UBQ gene as an internal control…
[HTML][HTML] … cotton nonexpressor of pathogenesis-related 1 (NPR1)-like genes family: evolutionary analysis and putative role in fiber development and defense pathway
… impact of GhNPR genes on fiber development, qRT-PCR was … Additionally, for the proper
development of the plant, its … Internal control gene GhUBQ4 was used to normalize the …
development of the plant, its … Internal control gene GhUBQ4 was used to normalize the …
[HTML][HTML] Phenylpropanoid metabolism and pigmentation show divergent patterns between brown color and green color cottons as revealed by metabolic and gene …
Z Li, Q Su, M Xu, J You, AQ Khan, J Li, X Zhang… - Journal of Cotton …, 2020 - Springer
… the reference gene to normalize gene expression … qRT-PCR was used to detect the expression
levels of lignin and lignan biosynthetic pathway genes in these three types of cotton fibers …
levels of lignin and lignan biosynthetic pathway genes in these three types of cotton fibers …
Cotton green fiber promotes suberin synthesis interfering cellulose deposition in the secondary cell wall
Z Chen, Y Li, Z Teng, Y Zhang, Y Liu, Q Suo… - Industrial Crops and …, 2023 - Elsevier
… ), cotton fiber is regarded as an ideal model for plant cell … Moreover, qRT-PCR analysis
indicated that Gh_D11G3168 … genes in Gossypium hirsutum for accurate normalization of …
indicated that Gh_D11G3168 … genes in Gossypium hirsutum for accurate normalization of …
[HTML][HTML] Over-expression of GhACTIN1 under the control of GhSCFP promoter improves cotton fiber and yield
… Cotton fiber provides a good model for studying cell elongation … Data normalization was
done using GAPDH as internal control … . The qRT-PCR analysis in advance generation (T 1 ) at …
done using GAPDH as internal control … . The qRT-PCR analysis in advance generation (T 1 ) at …
[HTML][HTML] Insights into the cotton anther development through association analysis of transcriptomic and small RNA sequencing
J Chen, P Su, P Chen, Q Li, X Yuan, Z Liu - BMC plant biology, 2018 - Springer
… important crops due to its fiber used as the principal natural … series of gene expression
for the gene after Log normalized … qRT-PCR analysis of the expression of miRNAs (ghr-miR393…
for the gene after Log normalized … qRT-PCR analysis of the expression of miRNAs (ghr-miR393…
Core cis‐element variation confers subgenome‐biased expression of a transcription factor that functions in cotton fiber elongation
… (qRT-PCR), with cotton histone-3 (AF024716) as the internal … The value of LUC was
normalized to that of REN. Promoter … raimondii, the best extant model of the D-genome progenitor, …
normalized to that of REN. Promoter … raimondii, the best extant model of the D-genome progenitor, …
[HTML][HTML] Identification and profiling of microRNAs expressed in elongating cotton fibers using small RNA deep sequencing
Y Wang, Y Ding, JY Liu - Frontiers in Plant Science, 2016 - frontiersin.org
… known miRNAs were normalized to reads per 10 million (RPTM). In the four … (qRT-PCR)
analysis was used to quantify the transcription levels of the nine target genes during cotton fiber …
analysis was used to quantify the transcription levels of the nine target genes during cotton fiber …
GhERF41, a group IIIe ERF protein, participates in regulating cell wall synthesis in cotton fiber
Y Gao, Y Cui, S Li, M Feng, L Liu, W Xu… - Industrial Crops and …, 2023 - Elsevier
… genes in fiber development, we examined their spatiotemporal expression in different tissues
at various developmental stages via qRT-PCR… as the internal control for normalization. …
at various developmental stages via qRT-PCR… as the internal control for normalization. …
[HTML][HTML] Cell-specific clock-controlled gene expression program regulates rhythmic fiber cell growth in cotton
D Wang, X Hu, H Ye, Y Wang, Q Yang, X Liang… - Genome Biology, 2023 - Springer
… g Heatmap showing normalized gene expression values for top 10 marker genes in each …
To experimentally test this hypothesis, we used qRT-PCR to characterize the RNA expression …
To experimentally test this hypothesis, we used qRT-PCR to characterize the RNA expression …