… Because various abiotic and biotic stresses strongly influence cotton fiber yield and quality,
… qRT-PCR) and normalized by the 2 −ΔΔCt method using the UBQ gene as an internal control…

… impact of GhNPR genes on fiber development, qRT-PCR was … Additionally, for the proper
development of the plant, its … Internal control gene GhUBQ4 was used to normalize the …

… the reference gene to normalize gene expression … qRT-PCR was used to detect the expression
levels of lignin and lignan biosynthetic pathway genes in these three types of cotton fibers …

… ), cotton fiber is regarded as an ideal model for plant cell … Moreover, qRT-PCR analysis
indicated that Gh_D11G3168 … genes in Gossypium hirsutum for accurate normalization of …

… Cotton fiber provides a good model for studying cell elongation … Data normalization was
done using GAPDH as internal control … . The qRT-PCR analysis in advance generation (T 1 ) at …

… important crops due to its fiber used as the principal natural … series of gene expression
for the gene after Log normalized … qRT-PCR analysis of the expression of miRNAs (ghr-miR393…

… (qRT-PCR), with cotton histone-3 (AF024716) as the internal … The value of LUC was
normalized to that of REN. Promoter … raimondii, the best extant model of the D-genome progenitor, …

… known miRNAs were normalized to reads per 10 million (RPTM). In the four … (qRT-PCR)
analysis was used to quantify the transcription levels of the nine target genes during cotton fiber …

… genes in fiber development, we examined their spatiotemporal expression in different tissues
at various developmental stages via qRT-PCR… as the internal control for normalization. …

… g Heatmap showing normalized gene expression values for top 10 marker genes in each …
To experimentally test this hypothesis, we used qRT-PCR to characterize the RNA expression …