… naive status. For this purpose, since some protocols use feeder-free culture conditions and
others do it in a co-culture … results obtained from all these different conditions. We studied the …

… conditions for naive human cells that we examined induced comparable upregulation of
SVAs (mainly SVA-D) and LTR5_Hs integrants (Figure 1F). The transposcriptomes of naive cells …

… The culture media used in this study had been described for generating naïve human ES
cells [9,12,13]. We had anticipated that bovine embryos cultured in these media during the time …

… state and have been invaluable for definition of culture conditions to support naïve hESC. …
In summary, true human naïve pluripotent cells are possible to maintain in culture. However, …

… systematic characterization of genetically matched naive-like cells generated using different
culture conditions, we … features with some more closely resembling cells from human ICM. …

… naive pluripotency growth conditions — designated NHSM (naive human stem cell medium)
— … expansion of genetically unmodified human PSCs, in both MEF-containing and MEF-free …

… be thawed before plating naïve hPSCs. In our hands, MEFs support naïve hPSCs growth
better if MEFs … Naïve hPSCs on freshly thawed MEFs (1 day) show flat morphology and slight …

… We elected to test the ability of culture conditions that sustain human naive PSCs after
resetting in vitro to support de novo derivation from dissociated human ICMs without PICMI …

… The isolation of naïve human pluripotent stem cells offers a … We outline key unresolved
questions regarding naïve human … as a model system for human development and disease. …

… PSCs prior to their conversion into naive PSCs 4,5,6,7,8,… human preimplantation-like state
can be induced in somatic cells by directly shifting reprogramming cultures to naive conditions …