… focus on optimizing protein refolding from inclusion bodies [2,3,4]… Here we aimed to use of
the PHA synthase as fusion protein … lead to further cleavage of the target protein. Interestingly, …

… other proteins expressed in the cell, 3) Low levels of degraded expressed proteins in the form
of inclusion bodies… and there is a need for final purification of the target protein bands. If the …

… For the purification of peptides, which can be cleaved from … reporter MalE, and it targets IB
formation to the periplasm while … The resolubilized GST fusion protein displayed GST activity, …

… coli usually ends up in inclusion bodies formation. Refolding of … of the target proteins, ABD
with or without signal sequence has been used for expression of fusion protein. Subsequently, …

… the fusion protein need to be removed from the target protein … and the intein should be
avoided during protein expression [… -cleavage as a fusion tag to produce tag-free inclusion body …

… -2 or fusion protein expression that leaves a fusion tag. In this … The target protein was
expressed as inclusion bodies, and … with a small fusion tag (His 6 - and a factor Xa cleavage site) …

… tags, tested target proteins, and optimization strategies. … cleavage sites as linkers in order
to analyze CatIB fusion and … In conclusion, the design of fusion proteins for CatIB formation is …

… and ease of expression than AXX; hence, we focused the … the N-terminus of other fusion
proteins mentioned in this study. … showed that all fusion proteins were successfully cleaved. This …

… cpCasp2, no off-target cleavage could be observed. … fusion protein process) technology
fulfills all requirements for advanced purification for the production of recombinant proteins. The …

… The target fragment from the PCR product was extracted and digested with NdeI and EcoRI
and … 1B), indicating that the fused protein was expressed in the form of inclusion bodies. …