… to target gene-modified cells to tissues. Gene-marked … and fluorescent bead uptake in
CD45+Lin–HLA-DR+ cells. We refer … -fluorescent staining with antibodies against GFP (green) and …

… metabolic diseases affecting the central nervous system (CNS)… (ALS), receive hematopoietic
cell transplantation (HCT) from … The rationale for the use of HCT to treat these disorders is …

… Early studies using hematopoietic stem cell (HSC) … utilized to mediate the transfer of green
fluorescent protein (GFP) gene … more suitable to be used as cell vehicles targeting the brain …

… ex vivo gene therapy targeting hematopoietic stem cells using a … activity in the central nervous
system and visceral organs. … EGFP fluorescence was observed in the cerebral cortex and …

… the central nervous system by improving neurological function, … However, given the diversity
of stem cells and disease targets… Green fluorescent protein is widely used as a reporter gene …

… The central nervous system (CNS) is a very unique system … For an optimal reconstitution
of the hematopoietic system by … T cells were shown to target transplanted hematopoietic …

… circulating monocytes into the central nervous system (CNS). … The images were captured
with a fluorescent microscope (… to express and deliver GDNF to target neurons effectively. We …

… and fluorescent microscopy studies of the gene-modified … The method was improved to use
hematopoietic stem cells (… of using leucocytes for targeted delivery of recombinant genes that …

… Cx3cr1-/+ versus WT cells within hematopoietic cell lineages … replace endogenous microglia
with gene modified MLCs upon … sections, we used a fluorescence microscope Delta Vision …

… Among these tools, CRISPR-Cas9 has been widely adopted as it uses an RNA molecule
(gRNA) to target the nuclease (Cas9) to the desired genomic locus. Cas9 binding and the …