[HTML][HTML] Rationally reduced libraries for combinatorial pathway optimization minimizing experimental effort
… Vector pMJ1 for fluorescent protein synthesis was constructed according to the Standard
European Vector Architecture (SEVA… of replication and the default SEVA multiple cloning site. …
European Vector Architecture (SEVA… of replication and the default SEVA multiple cloning site. …
The pAblo· pCasso self-curing vector toolset for unconstrained cytidine and adenine base-editing in Gram-negative bacteria
E Kozaeva, ZS Nielsen… - Nucleic Acids …, 2024 - academic.oup.com
… The Standard European Vector Architecture (SEVA) is a user-friendly plasmid platform that
facilitates standardization in synthetic biology (49). We adopted the SEVA framework, based …
facilitates standardization in synthetic biology (49). We adopted the SEVA framework, based …
Solvent tolerance in bacteria: fulfilling the promise of the biotech era?
H Kusumawardhani, R Hosseini, JH de Winde - Trends in biotechnology, 2018 - cell.com
… ] and can therefore be identified as promising and advantageous platforms for the production
of such potentially toxic compounds, or for bioremediation. These bacteria can efficiently …
of such potentially toxic compounds, or for bioremediation. These bacteria can efficiently …
[HTML][HTML] Propagation of recombinant genes through complex microbiomes with synthetic mini-RP4 plasmid vectors
T Aparicio, J Silbert, S Cepeda… - BioDesign Research, 2022 - spj.science.org
… The result was a coherent toolset (pMATING platform) … oriT sequence, the standard Km R
gene of SEVA vectors, and the … part of the modularity of the SEVA architecture, the exchange of …
gene of SEVA vectors, and the … part of the modularity of the SEVA architecture, the exchange of …
Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes
I Benedetti, V de Lorenzo, PI Nikel - Metabolic engineering, 2016 - Elsevier
… The transition between planktonic and biofilm lifestyles of the platform strain … analyze
biofilm structure by microscopy, we first introduced either plasmid pSYedQ or the control vector …
biofilm structure by microscopy, we first introduced either plasmid pSYedQ or the control vector …
In vivo fluorine biocatalysis: Six enzymes in search of a cell factory
… putida could be an ideal platform to host the six enzymes that convert mineral fluoride into …
plasmids—brought about by the Standard European Vector Architecture (SEVA)7—made the …
plasmids—brought about by the Standard European Vector Architecture (SEVA)7—made the …
Grand challenges for industrializing polyhydroxyalkanoates (PHAs)
D Tan, Y Wang, Y Tong, GQ Chen - Trends in biotechnology, 2021 - cell.com
Polyhydroxyalkanoates (PHAs) are a diverse family of sustainable bioplastics synthesized by
various bacteria, but their high production cost and unstable material properties make them …
various bacteria, but their high production cost and unstable material properties make them …
[HTML][HTML] Contemporary tools for regulating gene expression in bacteria
… Modern tools for regulating gene expression allow construction of better production platforms
through fine-tuning of gene expression, dynamic regulation, and autonomous, burden-…
through fine-tuning of gene expression, dynamic regulation, and autonomous, burden-…
Synthetic biology approaches towards the recycling of metals from the environment
MJ Capeness, LE Horsfall - Biochemical Society Transactions, 2020 - portlandpress.com
… The initial assembly and generation of all vectors are carried out in standard E. coli cloning
… , with some of these being in situ platforms for field analysis without the need for a laboratory. …
… , with some of these being in situ platforms for field analysis without the need for a laboratory. …
[PDF][PDF] The ProUSER2. 0 toolbox: Genetic parts and highly customizable plasmids for synthetic biology in Bacillus subtilis
V Mol, KB Falkenberg, AS de la Maza… - … the metabolic potential …, 2022 - orbit.dtu.dk
… European Vector Architecture (SEVA) collection9. However, a caveat of the SEVA standard
… subtilis we provide a well characterized vector platform enabling the user to tailor the …
… subtilis we provide a well characterized vector platform enabling the user to tailor the …