… -deficient mice [15]. Uba5 is a specific Ufm1-activating enzyme, and ufmylation is greatly …
erythroid development in the bone marrow of knockout mice. The percentages of all types of …

… activating enzyme 5 (UBA5) activates UFM1 through an adenylation reaction that consumes
ATP. UFM1-conjugase 1 (UFC1), the E2 conjugating enzyme, retrieves UFM1 from UBA5 by …

… enzymatic reaction involving E1, the UFM1-activating enzyme (ubiquitin-like modifier-activating
enzyme 5, UBA5… For instance, Uba5 knockout mice exhibited severe anemia, followed by …

… UFM1 is activated by the E1 enzyme UBA5, which forms a high-energy thioester bond
between its catalytic cysteine and the exposed C-terminal glycine of UFM1. Activated UFM1 is …

… Mouse genetics studies also revealed the indispensable roles of this … essential for conjugation.
The mature UFM1 is activated by the homodimer of UBA5, a UFM1-specific E1 enzyme, …

… , UBA5, UFL1 and UFBP1, leads to defective erythropoiesis … In adult mice, acute ablation of
either UFL1 or UFBP1 causes … indispensable role of UFMylation in definitive erythropoiesis [7…

… ubiquitously expressed UFM1 system has an essential role in erythroid differentiation in
mice, … The Ufm1-activating enzyme Uba5 is indispensable for erythroid differentiation in mice. …

… enzymatic reaction involving E1, the UFM1-activating enzyme (ubiquitin-like modifier-activating
enzyme 5; UBA5); … The anemia phenotype of the Uba5-deficient mouse embryos can be …

… While the UFM1 activating and conjugating enzymes share … of the E1 enzyme (UBA5) at the
apex of the enzymatic cascade is … In particular, germline deletion of UBA5 in mice invoked in …

… -specific enzymes, namely, Ufm1-activating E1 enzyme Uba5, … the indispensable role of
ufmyation in erythropoiesis 20,21,22 . … Ufl1 and Ufbp1 conditional knockout mice, we frequently …