… activate the NO dioxygenase function of Escherichia coli flavohemoglobin. Steady-state and
… E and F-helices and switches an electron transfer gate formed by LysF7, GlnE7, and water. …

… of the Escherichia coli flavoHb-NOD [8,9]. However, in the resting state, the Fe(III)-flavoHb
binds … and the bound ‘Coulomb blockade’ water molecule poised between the FAD C8M and …

… Glyoxalases and persulfide dioxygenases are examples of … a Diiron centre region of the
NO reducing Escherichia coli … enzyme is the cytoplasmatic flavohaemoglobin, having as the …

… -detoxifying NO metabolic activity with the flavoHb in Escherichia coli (Gardner et al. 1998a;
… modeled on the E. coli ferric flavoHb with a bridging Coulomb blockade water molecule (W) …

… , NO-dioxygenase, and peroxynitrite isomerase can play a pivotal role in the overall … the
synthesis of flavohemoglobin which plays a key role in protecting bacterial cells against NO [74]. …

… Escherichia coli, different detoxification systems have been identified: flavohaemoglobin (Hmp)
NO denitrosylase/dioxygenase [… layers of waters surrounding a protein core. Additionally, …

… It is soluble in water (approximately 1.6 mM at 37 C and 1.94 … to the NO dioxygenase
activities of flavohaemoglobin 12 . … Thus, for example, the Escherichia coli flavohaemoglobin (…

Mycobacterium tuberculosis (Mtb) produces an unconventional flavohemoglobin (MtbFHb)
that carries a FAD-binding site similar to D-lactate dehydrogenases (D-LDH) and oxidizes D-…

… Hence, the Fhb, also known as NO-dioxygenase, can … in an ice bath and a boiling water bath
three times for 10 min. … washed twice with 1 mL of distilled water, and re-suspended in 1 mL …

… Trophozoites were harvested by chilling the tubes in an iced water bath for 1 h. Detached …
electron shuttles, and one flavohemoglobin (gFlHb) with nitric oxide dioxygenase activity [14,33…