… Since this spatial organization cannot be captured in 2D cell culture, high-throughput …
multicellular tumor spheroid (MTS) models are needed. This review will describe the current use of …

… and invasion were monitored for 9 and 3 d, respectively. Furthermore, fluorescent staining
with … spheroid models for high-throughput (HT) cancer drug-screening assays. The 3D tumor …

… (b) Schematic representation of a multicellular 3D spheroid cell culture. … remain to be
addressed before spheroids can be used in high-throughput screening. The main challenge is to …

… In this study, a high-throughput drug screening system with autofluorescent cells cultured in
3-D … in 3-D spheroids but not cells grown on 2-D plastic surface [43]. Clearly, 2-D monolayer …

… model for tumor research, high-throughput drug screening, … culture can achieve high-throughput
preparation of spheroids… and is suitable for high-throughput screening. Owing to their …

… Efforts to develop high-throughput screening (HTS) methodologies using cultured cells as
three-dimensional (3D) spheroids and organoids have gained momentum for drug discovery …

… (one spheroid/well) commonly used for high-throughput … of the short culture time needed to
form compact spheroids with no or a … using real multicellular spheroids (Supplementary Note). …

… , multicellular spheroid models with scalable, automation-compatible plate technology enabling
image-based applications ranging from high-throughput screening … co-culture model of …

… (HCS) has lagged behind high-throughput screening (HTS) … ) models and the most common
3D in vitro culture method in … with high-throughput low-resolution microscopy of spheroids, …

… , which rely on high-throughput screening of thousands of … generation of uniform multicellular
spheroids, their staining with … 3-D organoid in vitro cell culture models for high-throughput …