Illuminating the live-cell dynamics of hepatitis B virus covalently closed circular DNA using the CRISPR-tag system
J Ding, Z Yi, W Zai, M Wu, B Chen, Q Cai, X Zhang… - MBio, 2023 - Am Soc Microbiol
… To monitor the cccDNA in real time, we devised a tracking system … treatments with actinomycin
D and RNase A, while DNase I … is similar to that of chromatin, suggesting that rcccDNA–…
D and RNase A, while DNase I … is similar to that of chromatin, suggesting that rcccDNA–…
Dynamic chromatin technologies: from individual molecules to epigenomic regulation in cells
… force spectroscopy and single-molecule tracking methods. … The establishment and maintenance
of chromatin states … the real-time dynamics of fluorescently labelled chromatin effectors …
of chromatin states … the real-time dynamics of fluorescently labelled chromatin effectors …
Reciprocal regulation of chromatin state and architecture by HOTAIRM1 contributes to temporal collinear HOXA gene activation
… Real-time polymerase chain reaction (PCR) quantification … Following DNaseI treatment, 1
μg of RNA from each fraction … uncoupling of HOTAIRM1-regulated genes, we wondered if the …
μg of RNA from each fraction … uncoupling of HOTAIRM1-regulated genes, we wondered if the …
The impact of chromatin dynamics on Cas9-mediated genome editing in human cells
RM Daer, JP Cutts, DA Brafman… - ACS synthetic biology, 2017 - ACS Publications
… of open chromatin: high levels of DNase-hypersensitivity and … -treated luciferase in the
unsilenced chromatin state showed … Real-time quantitative PCR reactions (15 μL each) contained …
unsilenced chromatin state showed … Real-time quantitative PCR reactions (15 μL each) contained …
High-throughput single-cell ChIP-seq identifies heterogeneity of chromatin states in breast cancer
K Grosselin, A Durand, J Marsolier, A Poitou… - Nature …, 2019 - nature.com
… In contrast, only a few studies have tracked the clonal evolution … 5a,b), which enables, in
three steps, the controlled production of … chromatin was then treated with RNase A, DNase and …
three steps, the controlled production of … chromatin was then treated with RNase A, DNase and …
Capsid-mediated chromatin state of the AAV vector genome controls host species range
… of vector copies in DNase I treated vs untreated nuclear … between species by treating
transduced cells with DNase I prior … from degradation in the +DNase I condition in both species. …
transduced cells with DNase I prior … from degradation in the +DNase I condition in both species. …
Chromatin states define tumour-specific T cell dysfunction and reprogramming
M Philip, L Fairchild, L Sun, EL Horste, S Camara… - Nature, 2017 - nature.com
… Control mice were treated with PBS and/or DMSO. … Additional PCR cycles were evaluated
by real time PCR. Final … were combined to create one signal track for each sample type. …
by real time PCR. Final … were combined to create one signal track for each sample type. …
Chromatin states shape insertion profiles of the piggyBac, Tol2 and Sleeping Beauty transposons and murine leukemia virus
J Yoshida, K Akagi, R Misawa, C Kokubu, J Takeda… - Scientific reports, 2017 - nature.com
… insertion sites and DNase I hypersensitive sites (DNase I HSs… were quantified by real-time
PCR using LightCycler FastStart DNA … We estimated the probability of success using control …
PCR using LightCycler FastStart DNA … We estimated the probability of success using control …
Chromatin states responsible for the regulation of differentially expressed genes under 60Co~γ ray radiation in rice
X Pan, Y Fang, X Yang, D Zheng, L Chen, L Wang… - BMC genomics, 2017 - Springer
… of up-regulated genes, but only chromatin states, instead of any … assess IR-related changes
in real-time transcription genome-wide, … After DNaseI treatment, the total RNA was reversely …
in real-time transcription genome-wide, … After DNaseI treatment, the total RNA was reversely …
Synthetic genome readers target clustered binding sites across diverse chromatin states
… track of the polyamide not predicted to be bound as a control. (… DNase HS sites and chromatin
states, whereas 2 and 4 show … preference for DNase HS sites or specific chromatin states. …
states, whereas 2 and 4 show … preference for DNase HS sites or specific chromatin states. …