… , derived principally from the Escherichia coli model (Fig. 1): … of sequence elements and
critical dimensions: It encodes an … substrates resulted in release of all transcripts. These results …

… from poly dAT, the rate of synthesis and release of 3-mer RNA (… RNA products are identified
by size and were quantified from … enzyme was performed as previously described using E. …

… (3, 4), release of short “abortive transcripts” and repositioning … enough strain is built in the
enzyme, the σ70 undergoes structural … quenched kinetics transcription assay that measures the …

… strategically engineered into Escherichia coli or Thermus … proportional in size to the length
of the transcribed template, … the transcription bubble and driving RNA release and bubble …

… It is currently unknown how the release of pyrophosphate affects transcription… enzyme with
purified σ S -his protein (about 1:3 molar ratio) at room temperature for 15 min followed by size…

… to further RNase E cleavage due to the preference of this enzyme for … These rate measurements
demonstrated that … treatment would release the cap from transcripts that had originally …

… release curve as measured in a coupled enzyme reaction … due to the reaction catalyzed by
the enzyme in question [43]. … for pyrophosphate release may occur at termination sites and …

… measured along with free mRNA as the total mRNA level, n r . … Our model of transcription
speed is robust against violations … supercoiling is released by the ATP-dependent enzyme DNA …

… Each expressed protein released peptides of intact N-termini that included an N-terminal …
We measured nanoluciferase expression under transcription control of the RhaP BAD …

… Instead of promoting constitutive transcription termination at the end of genes or operons,
regulated Rho-dependent terminators operate with tunable efficiency based on the presence …