… Collect Bosc23 medium (containing ecotropic retroviral particles) using a 25-mL serological
… retroviral vector carrying a GFP reporter (MOI = 20). 2 days after transduction, GFP + cells …

… and embryonic stem cells (SC), and induced pluripotent stem … puromycin retroviral vector
(addgene Plasmid#26358; [39]). … Phoenix-Amphotropic cells (or Ecotropic cells for experiments …

… by replication-defective viral vectors derived from the human immunodeficiency virus type 1
(… Thus, even when cells stably transduced by an HIV-1-based vector are transfected with the …

… site of the pBABE-Puro retroviral vector in the correct orientation … into PlatE cells, an ecotropic
packaging cell line.23 … in our experiments, because retroviral transduction did not induce …

… based on a retroviral vector incorporating a translational stop … and characterization of
embryonic stem cells, or the lengthy … empty control vector into NIH/3T3 fibroblasts transduced with …

… subcloned into the pMIG retroviral vector upstream of the … ; B B cells, T T cells, ES embryonic
stem cells. Expression level … Haemopoietic progenitor cells were isolated, transduced, and …

… patient’s somatic cells through the use of gene transduction of … characteristics as those of
embryonic stem cells (ESCs) 24 . … retroviral structure protein, including gag, pol, and ecotropic …

… differentiated somatic cells into induced pluripotent stem cells (iPSC… -transduction of target
cells with two different vectors expressing … vector (eg, ecotropic) could be considered for vector …

… Adenovirus vector is a kind of viral vector used often for gene … Embryonic stem cells (ESCs)
differentiate into … -10 (IL-10) through adenoviral transduction [10]. Transplantation of hypoxia-…

… we transduced SW13, a cell line which does not express keratin filaments, with recombinant
retroviruses … supernatants containing ecotropic viruses were used to transduce PT67 cells in …