… However, due to lack of therapy ER negative BC demands to … ) and was subjected to cDNA
synthesis using superscript II … Post 24 h transfection cells were treated with 100 nM E2 and …

… first estrogen receptor was cloned and characterized in the eighties of the last century from
cDNA of the human breast cancer cell line … , ovary cell transiently transfected with ERα cDNA …

… by adding distilled water instead of cDNA. To verify the identity of … of this receptor, we chose
this cell line for knockdown of ERβ. … ERβ knockdown cells together with cells transfected with …

… may depend on the hormone receptor status of breast cancer. … as well as the human
ER-negative (10) MDA-MB-231 cell line… tested by transfection of a pCMV6-XL5-PTEN vector into …

… We further tested the role of the estrogen receptor by genetic … We transfected MCF-7 cell
with either MIAT siRNA or a non-… functions in ER-negative breast cancer cell lines. Forced over-…

… in ER-positive cells compared with ER-negative cells. However, the expression levels of
eIF3a were quite similar among cell lines, … A, MCF7 cells transfected with scrambled siRNA or …

… with estrogen receptor (ER)α. However, it is an orphan … loss of function experiments using
ER-negative cell lines. We … was confirmed 24 h after transfection in both cell lines, while the …

… Using stably transfected MCF-7 cells, we showed that … with poor patient outcomes in
ER-negative breast cancer as well … the corresponding cDNA fragments into pDEST15 following the …

… A cDNA was first cloned from MCF-7 cells in 1985 and was … in ER-negative SK-BR-3 and
MDA-MB-231 cells [115]. … endogenous GATA3 in transfected MCF-7 cells. TCF7L2 functioned …

… of nuclear ER-positive cells, but nuclear ER-negative cells … We PCR-amplified the Elk-1
cDNA sequence encoding 327–… transfected this fusion protein GFP-ER(ΔNLS) or the GFP-ER(…