… determinants guiding enzyme activity in the superfamily. Its divergence contributes not
only to … , but also suggests different roles in the context of different enzymes of the family [70,71]. …

… unexpected because a phenylalanine at this position is important in another NSAR/OSBS
enzyme… We previously showed that the divergence of the other OSBS subfamilies was caused …

… is the product of divergent evolution and originated from a … the bifunctional enzymes with
OSBS and NSAAR activity have … are fundamental for the enzyme activity. Progress in the field …

… NSAR/OSBS subfamily to diverge from the rest of the OSBS family, lacks detectable NSAR
… and the unexpected pH-rate profile of the wild type enzyme’s NSAR activity made evaluating …

… mutations on function and specificity are often unpredictable, … The OSBS family of enzymes
is divided into several divergent … is highly divergent in sequence, but all the enzymes in the …

… In LvNSAR/OSBS, the R266Q mutation unexpectedly … in the NSAR/OSBS enzymes, and
it was surprising to see that it … of sequence divergence of the NSAR/OSBS enzymes in this …

… and functional assignment for enzymes … divergent evolution including the structural bases
for the evolution of new activities and the driving role of chemistry in it, and a model for enzyme …

… activity important for fitness, gene duplication followed by divergence can allow evolution of
a new enzyme … stumbled upon based upon unexpected phenotypes of mutant organisms. …

… Unexpectedly, however, the more conservative substitution to Phe led to … the divergence of
new enzymes by turning a latent, promiscuous activity into a physiologically relevant function, …

… favors the appearance of promiscuous enzymes in species inhabiting fluctuating environments,
as well as favors duplication events that allow functional divergence in enzymes. Thus, it …