… By contrast, the TaqMan assay, also known as fluorogenic 5′ nuclease assay, quantifies
through the addition of a probe labeled with fluorescent molecules that emit fluorescence …

… Thus, this study aimed to evaluate the diagnostic … TaqMan fluorogenic-labeled probes that
exploit the 5′ nuclease … Pearson correlation coefficient (r) was used in this study to assess …

… Moreover, the value of the unknown target is normalized to an … in one tube or to the use
of multiple fluorogenic probes for the … Real -time PCR assays have been developed for SNP …

… By contrast, the TaqMan assay, also known as fluorogenic 5′ nuclease assay, quantifies
through the addition of a probe labeled with fluorescent molecules that emit fluorescence …

… A 5' nuclease PCR assay for detection of the Y. pestis has … -PCR technique targeting the
caf1 gene was evaluated for … and Eisen RJ: Pentaplex real-time PCR for differential detection of …

… methods based on the polymerase chain reaction due to the … and in the system, which uses
MGB probes in multiplex mode [… using fluorogenic probes and the 5’ nuclease assay. Genetic …

… real-time PCR test combines 5′ nuclease technology with differentially labeled fluorogenic
… Five microliters of the template were used for each of the five multiplex PCR assays. An …

… We undertook the present comparative study to evaluate the performance of several … these
cover all real-time RT-PCR targets used in this study. We also used RNA extracted from the …

… development of the polymerase chain reaction (PCR) in the … (Roche Molecular Systems Inc.)
5′ nuclease assay [12, 19] … 3.3 cycles (1 log value) lower than the C Q value of the NTC [21]…

… , the 5′ nuclease activity of the DNA polymerase hydrolyzes … used in the optimization of the
multiplex real-time PCR assay… Evaluation of the developed multiplex real-time PCR assay in …