… for robust gene expression analysis. We encourage employing these novel reference genes
in … and NbGADPH) and NbPP2a, a previously validated reference gene for virus-infected N. …

… To analyze the differential expression of the 13 candidate reference genes in N. benthamiana
… Ct values of three biological replicates of each virus-infected group and the Mock infection …

… benthamiana infected by five viruses were quite different … expression, or the extent of variation
between experiments. Here we have validated several reference genes for use in tobacco …

… Differential gene expression analysis … To validate the RNA-seq data, 12 genes were
selected randomly for RT-qPCR … were found to be significantly regulated in virus-infected N. …

… genes, which need to be reactivated in the virus-infected … The x-axis shows the log2
transformed gene expression fold … were validated by qPCR analysis of selected genes (Fig. 1c). …

… reliability of the reference genes. This study provides useful information for gene expression
analysis during PNRSV infection and will facilitate gene expression studies associated with …

… have not been investigated in tobacco-PVY interaction either. This study aimed to identify
the most suitable reference genes for miRNA and mRNA expression analysis in tobacco …

… Among the si and mi samples indexed by RT-PCR respectively as virus infected and virus
… of reference genes is the validation by RT-qPCR analysis of a well-studied sugarcane gene …

… validate our findings, using two of the most stable and the one least stable validated reference
genes, one defense responsive gene … in virus-infected monocots using quantitative real-…

… the stability and validate suitable reference genes under a given … Lower M value indicates
higher stability in gene expression. … and viral RNAs in the virus-infected plants are mainly the …