… leveraged to perform targeted mutagenesis and disruption of … ACIAD0135, by transforming
an OE-PCR product containing the … in the strains we created could be combined in future work …

… be created through primers with polymerase chain reaction (PCR). The DNA … strain to
validate their causality. The process can be laborious, especially when there is a large mutation …

… an unsuccessful transformation would derive a PCR amplicon of … transformation stimulation
phenotype is linked to mutagenic … mutagenic concentrations enhanced natural transformation …

… created using the above natural transformation workflow. A single set of mutagenic primers
was … These insulators were added by successive PCR mutagenesis via overhangs to create …

… exceptional efficiency of natural transformation and allelic replacement in ADP1, an ideal …
This new strain, ADP1-ISx, demonstrated reduced mutation rates and higher transformation …

… Its high efficiency of natural transformation and allelic … by direct addition of the purified
PCR product to the exponentially … Although the mutation-transformed cells could be directly …

… If, on the other hand, a strain with no PCR product remained kanamycin … unanticipated
improvement in the efficiency of natural transformation. … Naturally transformable Acinetobacter sp. …

… For example, although we tout the plasticity of strain ADP1 and … carrying a known mutation
from the evolved strain, were … -transformation of different mutated alleles, each PCR product …

… To achieve high yields of microbial biofuel production using Acinetobacter baylyi ADP1,
we constructed and expressed two three-gene operons in E. coli K19 and A. baylyi …

… twitching in the bacterium Acinetobacter baylyi (strain ADP1, BD413). … Location of the mutation
was verified by PCR using … We tested at least one knockout mutation in each of the eleven …