… The genotype of all the constructions was verified using PCR and phenotype tests. All …
This is likely due to the complex nature and combined inhibitors in the SLH. Taken together, …

… oxidation process as well as site-directed mutagenesis to prove the role of putative catalytic
… Start Green PCR master mix, and a colony of Acinetobacter sp. O153 cells. PCR conditions …

… or pmrABC mutation mechanisms … PCR using the primers glmS F and Tn7R listed in Table
2. The expression of the inserted gene was confirmed using qRT-PCR before using the strain …

… Other deviations in Acinetobacter spp. from the general … region of ADP1 in strains
JH100-JH104, PCR products were … The mutation in strain JH104 was designed to mutate part of …

… Traditional random mutation strategies require a detectable … baylyi ADP1 contains no
known pathogenicity or virulence … Acinetobacter sp., including the close relative Acinetobacter …

… Transposon mutagenesis is coupled with downstream steps for … Transformation of such PCR
chimera allows the first … ) showed two CRISPR-cas in Acinetobacter spp. The first system is …

… -kanR cassette by OE-PCR and transformed to 130 A. baylyi … baylyi 156 ADP1 using natural
transformation. All plasmid … , 520 and site‐directed mutagenesis of two polyketide synthases. …

… Acinetobacter baylyi ADP1 exhibits high competency, facilitating natural transformation
55 … mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT 552 …

… observed when Acinetobacter baylyi ADP1 was exposed to … can affect natural transformation
frequency by mutagenic-… by quantitative reverse transcription PCR. Genes that were …

… baylyi ADP1, we extracted plasmids from those transformants and conducted PCR assays
of two … the combined amplify factor. All parameters used in the models are shown in Table 1. …