… Mutation of tfpB reduced transformation rates by two orders of magnitude, similar to the pilB
mutant; however, natural transformation … In-frame deletions were confirmed by PCR using …

… For Acinetobacter spp., most of the studies focusing on this … designed for retrotranscription
coupled to quantitative PCR (RT-… We next studied natural transformation kinetics by monitoring …

… on clinical manifestation are described for Acinetobacter spp. infections since most of the …
The EcoRI restriction site present in the lacI-tdk cassette was removed by PCR mutagenesis …

… natural transformation experiments with one particular … pathogenic strain harboring a genetic
mutation known to reduce … pathogenic from nonpathogenic Acinetobacter spp., as well as …

… thermal cycling program was used for all the PCR reactions, consisting of an initial 173 …
natural transformation followed by standard transposition of the entire IS26-composite 554 …

… , we identified two tigecycline-resistant Acinetobacter sp. … There was a mutation of the 76th
base from C to T in tet(X3.7). … Using inverse PCR, it was shown that the chromosome of A. …

… Real-time PCR demonstrated integrase dependent GI excision… alleles and suicide vectors
for mutagenesis of targeted A. … baylyi ADP1 can be transformed with a foreign DNA easily …

… This PCR product was used to transform ACN1276. The … Natural transformation and
chromosomal incorporation of DNA … we used site-directed mutagenesis to alter amino acids …

… suppressor mutation in pldA is more likely a causal mutation … baylyi strains were derived
from ADP1 and grown in LB-… /lptD) was PCR amplified from respective strains and gel purified. …

… deletion strains were constructed by targeted mutagenesis … deletion mutant strains were
made through natural transformation of … All deletion constructs were confirmed by PCR. Insertion …