De Novo Biosynthesis of Glutarate via α-Keto Acid Carbon Chain Extension and Decarboxylation Pathway in Escherichia coli

J Wang, Y Wu, X Sun, Q Yuan, Y Yan - ACS synthetic biology, 2017 - ACS Publications
α-ketoglutarate (α-KG) in combination with α-keto acid decarboxylation pathway in Escherichia
coli… ) and homoisocitrate dehydrogenase (HICDH) from Saccharomyces cerevisiae into E. …

Metabolic engineering of cofactor flavin adenine dinucleotide (FAD) synthesis and regeneration in Escherichia coli for production of αketo acids

Y Hou, GS Hossain, J Li, HD Shin, G Du… - Biotechnology and …, 2017 - Wiley Online Library
… In this study, a system involving the synthesis pathway and regeneration of FAD was
engineered in Escherichia coli to improve αketo acid production—from the corresponding l‐amino …

Biotechnological production of alpha-keto acids: current status and perspectives

Y Song, J Li, H Shin, L Liu, G Du, J Chen - Bioresource technology, 2016 - Elsevier
… Although most α-keto acids are currently produced by … first present the different types of
α-keto acids as well as their … α-keto acids; namely, pyruvate, α-ketoglutarate, α-ketoisovalerate, …

Engineering of L-amino acid deaminases for the production of α-keto acids from L-amino acids

P Nshimiyimana, L Liu, G Du - Bioengineered, 2019 - Taylor & Francis
… In the laboratory, when using Escherichia coli cells expressing D-2-hydroxyisocaproate
dehydrogenase from Lactobacillus delbrueckii and formate dehydrogenase from Mycobacterium …

Characterisation of a thiamine diphosphate-dependent alpha-keto acid decarboxylase from Proteus mirabilis JN458

B Wang, Y Bai, T Fan, X Zheng, Y Cai - Food chemistry, 2017 - Elsevier
… Alpha-keto acid decarboxylases can convert keto acids to their … α-keto acid decarboxylase
in Proteus mirabilis JN458 was cloned, and the enzyme overexpressed in Escherichia coli

Hydroxytyrosol production from l-DOPA by engineered Escherichia coli co-expressing l-amino acid deaminase, α-keto acid decarboxylase, aldehyde reductase and …

J Huo, Y Bai, TP Fan, X Zheng, Y Cai - Process Biochemistry, 2022 - Elsevier
… The recombinant Escherichia coli cells were constructed by co-expressing L-amino acid …
, α-keto acid decarboxylase from Proteus mirabilis, aldehyde reductase from Escherichia coli (…

Efficient enzymatic synthesis of α-keto acids by redesigned substrate-binding pocket of the L-amino acid deaminase (PmiLAAD)

L Wu, X Guo, G Wu, P Liu, Z Liu - Enzyme and microbial technology, 2020 - Elsevier
… This study has developed a robust whole-cell E. coli biocatalyst for α-keto acids production
by protein engineering, and this strategy may be useful for the construction of other …

Production of α‐ketobutyrate using engineered Escherichia coli via temperature shift

C Zhang, J Qi, Y Li, X Fan, Q Xu… - Biotechnology and …, 2016 - Wiley Online Library
… Alpha-keto acid belongs to a class of bifunctional chemical organic compounds. It serves
as an active intermediate in organic synthesis and biological metabolism; and is often used in …

… biosynthesis: stable functional expression of branched chain α-keto acid dehydrogenase complex from Streptomyces avermitilis in Escherichia coli by selectively …

Q Cui, F Zhou, W Liu, Y Tao - Biotechnology letters, 2017 - Springer
α-keto acid dehydrogenase complex (BKDH) from Streptomyces avermitilis was systematically
optimized by selectively regulating individual subunit expression in Escherichia coli. …

Improving methyl ketone production in Escherichia coli by heterologous expression of NADH‐dependent FabG

EB Goh, Y Chen, CJ Petzold… - Biotechnology and …, 2018 - Wiley Online Library
… We previously engineered Escherichia coli to overproduce medium- to long-chain saturated
and monounsaturated methyl ketones, which could potentially be applied as diesel fuel …