Automatic control of gene expression in mammalian cells

C Fracassi, L Postiglione, G Fiore… - ACS synthetic …, 2016 - ACS Publications
… control gene expression from the tetracycline-inducible promoter in mammalian cells in real
time… We validated our method in a monoclonal population of cells constitutively expressing a …

[HTML][HTML] Light-mediated control of Gene expression in mammalian cells

M Yamada, SC Nagasaki, T Ozawa, I Imayoshi - Neuroscience Research, 2020 - Elsevier
gene expression patterns/changes and cellular/tissue functions, we need to systematically
survey cellular gene expression … develop tools to manipulate gene expression at fine spatial …

Ultrasound imaging of gene expression in mammalian cells

A Farhadi, GH Ho, DP Sawyer, RW Bourdeau… - Science, 2019 - science.org
… Here, we describe the expression of ARGs in mammalian cells to enable ultrasound imaging
of mammalian gene expression. To identify a set of genes capable of encoding gas vesicle …

[HTML][HTML] Precise tuning of gene expression levels in mammalian cells

YS Michaels, MB Barnkob, H Barbosa… - Nature …, 2019 - nature.com
… (miRNAs) to fine-tune gene expression in mammalian cells. miRNAs are short non-coding …
gene expression levels by recruiting the RNA induced silencing complex (RISC) to cellular

[HTML][HTML] Characterization and mitigation of gene expression burden in mammalian cells

T Frei, F Cella, F Tedeschi, J Gutiérrez, GB Stan… - Nature …, 2020 - nature.com
… of genetic networks in mammalian cells is still painstakingly slow and fraught with
inexplicable failures. Here, we demonstrate that transiently expressed genes in mammalian cells

Fundamentals of expression in mammalian cells

MR Dyson - Advanced technologies for protein complex production …, 2016 - Springer
… plasmids have been developed for transient gene expression (TGE) containing the SV40
or EBNA1 origins of replication [14] to aid episomal replication of plasmids and potentially …

Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics

S Gutiérrez-Granados, L Cervera… - Critical reviews in …, 2018 - Taylor & Francis
Transient gene expression (TGE) in animal cell cultures has been used for almost 30 years
to produce milligrams and grams of recombinant proteins, virus-like particles and viral vectors…

[HTML][HTML] A synthetic transcription platform for programmable gene expression in mammalian cells

WCW Chen, L Gaidukov, Y Lai, MR Wu, J Cao… - Nature …, 2022 - nature.com
… Our group has also developed crisprTFs to regulate gene expression driven by natural or …
system for the programmable regulation of gene expression in mammalian cells. Our goal is to …

Synthetic control systems for high performance gene expression in mammalian cells

G Lillacci, Y Benenson, M Khammash - Nucleic acids research, 2018 - academic.oup.com
… We introduce four cybergenetic circuits for controlling inducible gene expression in
mammalian cells: an open loop circuit (OLP), a negative feedback controller circuit (FBK), an IFF …

Gene expression in mammalian cells using BacMam, a modified baculovirus system

JA Fornwald, Q Lu, FM Boyce, RS Ames - … and Insect Cell Expression …, 2016 - Springer
… stable mammalian cell line … for mammalian gene expression. Although virus generation
requires an additional 2 weeks for reagent preparation in comparison to mammalian expression