High efficient multisites genome editing in allotetraploid cotton (Gossypium hirsutum) using CRISPR/Cas9 system

P Wang, J Zhang, L Sun, Y Ma, J Xu… - Plant biotechnology …, 2018 - Wiley Online Library
… system in allotetraploid cotton and accomplished multiple sites genome editing. The gene
DsRed2 was chosen as a target. Firstly, an DsRed2-overexpressed transgenic cotton line, …

[HTML][HTML] Robust CRISPR/Cpf1 (Cas12a)‐mediated genome editing in allotetraploid cotton (Gossypium hirsutum)

B Li, H Rui, Y Li, Q Wang, M Alariqi, L Qin… - Plant biotechnology …, 2019 - ncbi.nlm.nih.gov
… The editing extent per plant was mainly concentrated in 20%… system has high gene editing
efficiency in cotton. Most of the … a site genome editing in allotetraploid cotton with 87% editing

High‐efficient and precise base editing of C•G to T•A in the allotetraploid cotton (Gossypium hirsutum) genome using a modified CRISPR/Cas9 system

L Qin, J Li, Q Wang, Z Xu, L Sun… - Plant biotechnology …, 2020 - Wiley Online Library
… Comparing the off-target effects in previous reports, the GhBE3 system used in this report
is highly precise for cotton genome editing. The negligible C→T conversions at these off-target …

CRISPR/Cas9: A New Genome Editing Tool to Accelerate Cotton (Gossypium spp.) Breeding

MN Sattar, Z Iqbal, SD Dangol, A Bakhsh - Advances in Plant Breeding …, 2019 - Springer
… Inadequate achievement in pioneering desired genes from diploid species to Gossypium
hirsutum is because of obscurity in the F1 hybrid production, chromosome number resurgence …

Cotton (Gossypium hirsutum L.) Breeding Strategies

S Rauf, M Shehzad, JM Al-Khayri, HM Imran… - Advances in Plant …, 2019 - Springer
… due to the absence of efficient genetic transformation (Long et al. 2018). The application
of CRISPR/Cas9 in cotton genome editing is detailed in a separate chapter in this book. …

Targeted mutagenesis in cotton (Gossypium hirsutum L.) using the CRISPR/Cas9 system

X Chen, X Lu, N Shu, S Wang, J Wang, D Wang… - Scientific reports, 2017 - nature.com
… -mediated targeted mutagenesis in cotton. Here, we report the genome editing and targeted
mutagenesis in upland cotton (Gossypium hirsutum L., hereafter cotton) using the CRISPR/…

Efficient genotype‐independent cotton genetic transformation and genome editing

X Ge, J Xu, Z Yang, X Yang, Y Wang… - Journal of Integrative …, 2023 - Wiley Online Library
… Upland cotton (Gossypium hirsutum L.) is one of the most widely cultivated and economically
valuable crops for the production of natural fibers for the textile industry and seeds for …

[HTML][HTML] Biotechnological solutions for major cotton (Gossypium hirsutum) pathogens and pests

R Tarazi, JLS Jimenez, MFS Vaslin - Biotechnology Research and …, 2019 - Elsevier
… The continuous development of cotton genome sequencing and genome editing technologies
associated with the improvement of transgenic technology and the use of marker-assisted …

Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)

G Wang, Z Xu, F Wang, Y Huang, Y Xin, S Liang, B Li… - BMC biology, 2022 - Springer
… sequencing analysis for the off-target effects in GhABE7.10-n/dCas9-edited cotton To
investigate the genome editing specificity of ABEs in cotton, two edited plants generated by the …

A high-efficiency CRISPR/Cas9 system for targeted mutagenesis in Cotton (Gossypium hirsutum L.)

C Li, T Unver, B Zhang - Scientific reports, 2017 - nature.com
… In this study, a set of CRISPR/Cas9 genome editing system was firstly proved to possess the
… allotetraploid cotton genome editing, which may extremely enhance cotton genomic study …