RNA-Seq: revelation of the messengers

MC Van Verk, R Hickman, CMJ Pieterse… - Trends in plant …, 2013 - cell.com
Next-generation RNA-sequencing (RNA-Seq) is rapidly outcompeting microarrays as the
technology of choice for whole-transcriptome studies. However, the bioinformatics skills …

[HTML][HTML] Catalyzing plant science research with RNA-seq

LBB Martin, Z Fei, JJ Giovannoni… - Frontiers in plant …, 2013 - frontiersin.org
Next generation DNA sequencing technologies are driving increasingly rapid, affordable
and high resolution analyses of plant transcriptomes through sequencing of their associated …

Discovering new biology through sequencing of RNA

APM Weber - Plant physiology, 2015 - academic.oup.com
Abstract Sequencing of RNA (RNA-Seq) was invented approximately 1 decade ago and has
since revolutionized biological research. This update provides a brief historic perspective on …

Designing a transcriptome next‐generation sequencing project for a nonmodel plant species1

SR Strickler, A Bombarely… - American journal of …, 2012 - Wiley Online Library
The application of next‐generation sequencing (NGS) to transcriptomics, commonly called
RNA‐seq, allows the nearly complete characterization of transcriptomic events occurring in …

[HTML][HTML] A comprehensive online database for exploring∼ 20,000 public Arabidopsis RNA-seq libraries

H Zhang, F Zhang, Y Yu, LI Feng, J Jia, BO Liu, B Li… - Molecular Plant, 2020 - cell.com
In the last decade, RNA-sequencing (RNA-seq) has surpassed microarray to become the
gold standard for gene expression profiling due to the continuous drop in sequencing cost …

RNA-Seq for transcriptome analysis in non-model plants

R Garg, M Jain - Legume Genomics: Methods and Protocols, 2013 - Springer
Sequencing of mRNA using next-generation sequencing (NGS) technologies (RNA-seq)
has the potential to reveal unprecedented complexity of the transcriptomes. The …

[HTML][HTML] Upcoming progress of transcriptomics studies on plants: An overview

P Tyagi, D Singh, S Mathur, A Singh… - Frontiers in Plant …, 2022 - frontiersin.org
Transcriptome sequencing or RNA-Sequencing is a high-resolution, sensitive and high-
throughput next-generation sequencing (NGS) approach used to study non-model plants …

An 'eFP‐Seq Browser'for visualizing and exploring RNA sequencing data

A Sullivan, PK Purohit, NH Freese, A Pasha… - The Plant …, 2019 - Wiley Online Library
Improvements in next‐generation sequencing technologies have resulted in dramatically
reduced sequencing costs. This has led to an explosion of '‐seq'‐based methods, of which …

[HTML][HTML] Optimization of an RNA-Seq differential gene expression analysis depending on biological replicate number and library size

S Lamarre, P Frasse, M Zouine, D Labourdette… - Frontiers in plant …, 2018 - frontiersin.org
RNA-Seq is a widely used technology that allows an efficient genome-wide quantification of
gene expressions for, for example, differential expression (DE) analysis. After a brief review …

[HTML][HTML] The bench scientist's guide to statistical analysis of RNA-Seq data

CR Yendrek, EA Ainsworth, J Thimmapuram - BMC research notes, 2012 - Springer
Abstract Background RNA sequencing (RNA-Seq) is emerging as a highly accurate method
to quantify transcript abundance. However, analyses of the large data sets obtained by …