RNAs are important biomolecules that play essential roles in various cellular processes and
are crucially linked with many human diseases. The key to elucidate the mechanisms …

Site-directed spin labeling (SDSL) of large RNAs for electron paramagnetic resonance
(EPR) spectroscopy has remained challenging to date. We here demonstrate an efficient …

Site-directed spin labeling (SDSL) is widely applied for structural studies of biopolymers by
electron paramagnetic resonance (EPR). However, SDSL of long RNA sequences still …

Site-directed spin labeling is emerging as an essential tool to investigate the structural and
dynamical features of RNA. We propose here an enzymatic method, which allows the …

RNA is a crucial regulator involved in most molecular processes of life. Understanding its
function at the molecular level requires high-resolution structural information. However, the …

The introduction of chemical modifications into long RNA molecules at specific positions for
visualization, biophysical investigations, diagnostic and therapeutic applications still …

In site-directed spin-labeling (SDSL), a stable nitroxide radical is attached to a specific
location within a macromolecule and electron paramagnetic resonance (EPR) spectroscopy …

Paramagnetic relaxation enhancement (PRE) NMR is a powerful method to study structure,
dynamics and function of proteins. Up to now, the application of PRE NMR on RNAs is a …

Ribonucleic acid function is governed by its structure, dynamics, and interaction with other
biomolecules and influenced by the local environment. Thus, methods are needed that …

In RNA site-directed spin labeling (SDSL) studies, structural and dynamic information at the
individual RNA nucleotide level is derived from the observed electron paramagnetic …