Background The regeneration of cofactors and the supply of alkane substrate are key
considerations for the biocatalytic activation of hydrocarbons by cytochrome P450s. This …

Background Biocatalyst improvement through molecular and recombinant means should be
complemented with efficient process design to facilitate process feasibility and improve …

CYP153A6 is a well-studied terminal alkane hydroxylase which has previously been
expressed in Pseudomonas putida and Escherichia coli by using the pCom8 plasmid. In this …

Some of the most promising results for bacterial alkane hydroxylation to alcohols have been
obtained with the cytochrome P450 monooxygenase CYP153A6. CYP153A6 belongs to the …

Several strains that grow on medium-chain-length alkanes and catalyze interesting
hydroxylation and epoxidation reactions do not possess integral membrane nonheme iron …

Medium-chain α, ω-alkanediols are extensively used as monomers in the polymer and
chemical industries, with most α, ω-alkanediol production dependent on chemical …

Systematic screening of single-gene knockout collection of Escherichia coli BW25113 (the
Keio collection) was performed to select mutants that could enhance the deethylation of 7 …

Cytochrome P450 monooxygenases (CYPs) are valuable biocatalysts for the
oxyfunctionalization of non‐activated carbon‐hydrogen bonds. Most CYPs rely on electron …

Cytochrome P450 monooxygenases (Cyps) effectively catalyze the regiospecific
oxyfunctionalization of inert C–H bonds under mild conditions. Due to their cofactor …

The first and key step in alkane metabolism is the terminal hydroxylation of alkanes to 1-
alkanols, a reaction catalyzed by a family of integral-membrane diiron enzymes related to …