Omics-driven identification and elimination of valerolactam catabolism in Pseudomonas putida KT2440 for increased product titer

MG Thompson, LE Valencia, JM Blake-Hedges… - Metabolic Engineering …, 2019 - Elsevier
Pseudomonas putida is a promising bacterial chassis for metabolic engineering given its
ability to metabolize a wide array of carbon sources, especially aromatic compounds derived …

Massively Parallel Fitness Profiling Reveals Multiple Novel Enzymes in Pseudomonas putida Lysine Metabolism

MG Thompson, JM Blake-Hedges, P Cruz-Morales… - MBio, 2019 - Am Soc Microbiol
Despite intensive study for 50 years, the biochemical and genetic links between lysine
metabolism and central metabolism in Pseudomonas putida remain unresolved. To …

[PDF][PDF] Massively parallel fitness profiling reveals multiple novel enzymes in Pseudomonas putida lysine metabolism. mBio 10

MG Thompson, JM Blake-Hedges, P Cruz-Morales… - 2019 - researchgate.net
Despite intensive study for 50years, the biochemical and genetic links between lysine
metabolism and central metabolism in Pseudomonas putida remain unresolved. To …

Fatty acid and alcohol metabolism in Pseudomonas putida: functional analysis using random barcode transposon sequencing

MG Thompson, MR Incha, AN Pearson… - Applied and …, 2020 - Am Soc Microbiol
With its ability to catabolize a wide variety of carbon sources and a growing engineering
toolkit, Pseudomonas putida KT2440 is emerging as an important chassis organism for …

Evolution and engineering of pathways for aromatic O-demethylation in Pseudomonas putida KT2440

AC Bleem, E Kuatsjah, J Johnsen, ET Mohamed… - Metabolic …, 2024 - Elsevier
Biological conversion of lignin from biomass offers a promising strategy for sustainable
production of fuels and chemicals. However, aromatic compounds derived from lignin …

Nitrogen metabolism in Pseudomonas putida: functional analysis using random barcode transposon sequencing

M Schmidt, AN Pearson, MR Incha… - Applied and …, 2022 - Am Soc Microbiol
Pseudomonas putida KT2440 has long been studied for its diverse and robust metabolisms,
yet many genes and proteins imparting these growth capacities remain uncharacterized …

Metabolic engineering of Corynebacterium glutamicum for the high-level production of valerolactam, a nylon-5 monomer

T Han, SY Lee - Metabolic Engineering, 2023 - Elsevier
Valerolactam (VL) is an important precursor chemical for nylon-5 and nylon 6, 5. It has been
produced by petroleum-based route involving harsh reaction conditions and generating …

Integrated Bioprocessing for the pH-Dependent Production of 4-Valerolactone from Levulinate in Pseudomonas putida KT2440

CH Martin, D Wu, KLJ Prather - Applied and environmental …, 2010 - Am Soc Microbiol
Enzymes are powerful biocatalysts capable of performing specific chemical transformations
under mild conditions, yet as catalysts they remain subject to the laws of thermodynamics …

Vanillin Production in Pseudomonas: Whole-Genome Sequencing of Pseudomonas sp. Strain 9.1 and Reannotation of Pseudomonas putida CalA as a Vanillin …

J García-Hidalgo, DP Brink, K Ravi… - Applied and …, 2020 - Am Soc Microbiol
Microbial degradation of lignin and its related aromatic compounds has great potential for
the sustainable production of chemicals and bioremediation of contaminated soils. We …

Eliminating a global regulator of carbon catabolite repression enhances the conversion of aromatic lignin monomers to muconate in Pseudomonas putida KT2440

CW Johnson, PE Abraham, JG Linger, P Khanna… - Metabolic engineering …, 2017 - Elsevier
Carbon catabolite repression refers to the preference of microbes to metabolize certain
growth substrates over others in response to a variety of regulatory mechanisms. Such …