Cytochrome P450s are a diverse and versatile class of enzymes, able to carry out
oxyfunctionalisation of hydrocarbons with exceptional regio-and stereospecificity. They …

Background The regeneration of cofactors and the supply of alkane substrate are key
considerations for the biocatalytic activation of hydrocarbons by cytochrome P450s. This …

Whole-cell biocatalysis is a preferred option in CYP450 catalyzed biotransformations,
because expensive nicotinamide cofactors are supplied and regenerated by cellular …

Systematic screening of single-gene knockout collection of Escherichia coli BW25113 (the
Keio collection) was performed to select mutants that could enhance the deethylation of 7 …

Large stockpiles of linear hydrocarbons have arisen as by-products from the global
expansion of gas-to-liquid refining processes. Furthermore, these linear alkanes feature one …

Cytochrome P450s (CYPs) are key enzymes involved in drug and xenobiotic metabolism. A
wide array of in vitro methodologies, including recombinant sources, are currently been …

CYP153A6 is a well-studied terminal alkane hydroxylase which has previously been
expressed in Pseudomonas putida and Escherichia coli by using the pCom8 plasmid. In this …

CYP102A1 is an efficient medium-to long-chain fatty acid hydroxylase that is able to accept
a wide range of non-natural substrates which bear no resemblance to the natural ones. 4 …

Cytochrome P450 monooxygenases are a superfamily of heme-containing enzymes that are
found in all domains of life. P450s catalyse diverse reactions, many of which are difficult …

Cytochrome P450 monooxygenases (CYPs) are valuable biocatalysts for the
oxyfunctionalization of non‐activated carbon‐hydrogen bonds. Most CYPs rely on electron …