Abstract Biotin (vitamin B 7, hexahydro-2-oxo-1H-thieno [3, 4-d] imidazole-4-pentanoic acid)
is not synthesized by mammals: dietary sources include plant based foods, yeast, liver …

Biotin (or Vitamin B7) is a vitamin where deficiency can be caused by inadequate intake.
Biotin deficiency is rare, as most people get enough biotin from diet, since many foods …

Methods of assessing biotin status in humans, while not welldelineated, are likely to include
measuring plasma and urine biotin response to an oral load of biotin. In order to successfully …

Background: Biotin is a water-soluble vitamin which plays an important biochemical role in a
variety of carboxylase-mediated metabolic reactions. Determination of biotin status for the …

Background: The results of clinical studies have provided evidence that marginal biotin
deficiency is more common than was previously thought. A previous study of 10 subjects …

In human urine, the biotin concentration assayed directly using an avidin-binding assay
(ABA) apparently overestimates “true” biotin concentration as measured by HPLC …

A method for measuring biotin by affinity-chromatography was developed using a trypsin-
treated avidin silica gel column. Elution was by a linear gradient of propan-2-ol in an acidic …

Despite sobering evidence of no benefit or even possible harm, the use of vitamin and
mineral supplements by adults continues to grow. It is estimated that the US supplement …

Biotin is a water-soluble vitamin and serves as a coenzyme for five carboxylases, which
catalyze key steps in the metabolism of fatty acids, glucose, and amino acids in humans …

Moreover, the finding of high bioavailability of biotin at pharmacologic doses provides at
least some basis for predicting that bioavailability will also be high at lower doses …