User-friendly, scalable tools and workflows for single-cell RNA-seq analysis

P Moreno, N Huang, JR Manning, S Mohammed… - Nature …, 2021 - nature.com
To the Editor—As single-cell RNA sequencing (scRNA-seq) becomes widespread,
accessible and scalable computational pipelines for data analysis are needed. We introduce …

[HTML][HTML] Exploring the single-cell RNA-seq analysis landscape with the scRNA-tools database

L Zappia, B Phipson, A Oshlack - PLoS computational biology, 2018 - journals.plos.org
As single-cell RNA-sequencing (scRNA-seq) datasets have become more widespread the
number of tools designed to analyse these data has dramatically increased. Navigating the …

[HTML][HTML] Single-cell RNA-seq technologies and related computational data analysis

G Chen, B Ning, T Shi - Frontiers in genetics, 2019 - frontiersin.org
Single-cell RNA sequencing (scRNA-seq) technologies allow the dissection of gene
expression at single-cell resolution, which greatly revolutionizes transcriptomic studies. A …

Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data

TS Andrews, VY Kiselev, D McCarthy, M Hemberg - Nature protocols, 2021 - nature.com
Single-cell RNA sequencing (scRNA-seq) is a popular and powerful technology that allows
you to profile the whole transcriptome of a large number of individual cells. However, the …

The triumphs and limitations of computational methods for scRNA-seq

PV Kharchenko - Nature methods, 2021 - nature.com
The rapid progress of protocols for sequencing single-cell transcriptomes over the past
decade has been accompanied by equally impressive advances in the computational …

ascend: R package for analysis of single-cell RNA-seq data

A Senabouth, SW Lukowski, JA Hernandez… - …, 2019 - academic.oup.com
Background Recent developments in single-cell RNA sequencing (scRNA-seq) platforms
have vastly increased the number of cells typically assayed in an experiment. Analysis of …

[HTML][HTML] Over 1000 tools reveal trends in the single-cell RNA-seq analysis landscape

L Zappia, FJ Theis - Genome biology, 2021 - Springer
Recent years have seen a revolution in single-cell RNA-sequencing (scRNA-seq)
technologies, datasets, and analysis methods. Since 2016, the scRNA-tools database has …

[HTML][HTML] Data analysis guidelines for single-cell RNA-seq in biomedical studies and clinical applications

M Su, T Pan, QZ Chen, WW Zhou, Y Gong, G Xu… - Military Medical …, 2022 - Springer
The application of single-cell RNA sequencing (scRNA-seq) in biomedical research has
advanced our understanding of the pathogenesis of disease and provided valuable insights …

[HTML][HTML] Comparative analysis of single-cell RNA sequencing methods

C Ziegenhain, B Vieth, S Parekh, B Reinius… - Molecular cell, 2017 - cell.com
Single-cell RNA sequencing (scRNA-seq) offers new possibilities to address biological and
medical questions. However, systematic comparisons of the performance of diverse scRNA …

[HTML][HTML] A systematic evaluation of single cell RNA-seq analysis pipelines

B Vieth, S Parekh, C Ziegenhain, W Enard… - Nature …, 2019 - nature.com
The recent rapid spread of single cell RNA sequencing (scRNA-seq) methods has created a
large variety of experimental and computational pipelines for which best practices have not …