Motivation: RNA-seq is replacing microarrays as the primary tool for gene expression
studies. Many RNA-seq studies have used insufficient biological replicates, resulting in low …

Advances in next generation sequencing (NGS) technologies resulted in a broad array of
large-scale gene expression studies and an unprecedented volume of whole messenger …

Single-cell RNA-sequencing analysis to quantify the RNA molecules in individual cells has
become popular, as it can obtain a large amount of information from each experiment. We …

Background Recent developments in single-cell RNA sequencing (scRNA-seq) platforms
have vastly increased the number of cells typically assayed in an experiment. Analysis of …

Global run-on sequencing (GRO-seq) is a recent addition to the series of high-throughput
sequencing methods that enables new insights into transcriptional dynamics within a cell …

Background Direct cDNA preamplification protocols developed for single-cell RNA-seq have
enabled transcriptome profiling of precious clinical samples and rare cell populations …

Next-generation sequencing technologies are now being exploited not only to analyse static
genomes, but also dynamic transcriptomes in an approach termed RNA-seq. Although these …

RNA-seq has revolutionized the research community approach to studying gene expression.
In fact, this technology has opened up the possibility of quantifying the expression level of all …

The devil in the details of RNA-seq | Nature Biotechnology Skip to main content Thank you for
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Gene expression is the fundamental level at which the results of various genetic and
regulatory programs are observable. The measurement of transcriptome-wide gene …