Materials and Methods Unless specified otherwise, all chemicals were supplied from
Millipore Sigma (St Louis, MO, USA. Nonessential amino acids (NEAA), essential amino …

The first culture media designed specifically to support development of the preimplantation
mouse embryo were formulated over 50 years ago and were based on balanced salt …

Further refinement of culture media is needed to improve the quality of embryos generated
in vitro. Previous results from our laboratory demonstrated that uptake of nutrients by the …

The preimplantation period is a unique time during mammalian development when an
embryo composed of between one and a few thousand cells survives in the absence of any …

Background Whole embryo culture is a valuable research method in mammalian
developmental biology and birth defects research, enabling longitudinal studies of …

Objective To determine the composition of commercially available culture media and test
whether differences in composition are biologically relevant in a murine model. Design …

Since the inception of modern embryo culture media over 50 years ago there have been
significant developments in culture systems for the mammalian preimplantation embryo …

The culture of preimplantation embryos in vitro is an important method for human and mouse
reproductive technology. This study aims to investigate the influence of different conditions …

Efficient model production in rats that incorporates newly developed genetic editing and
embryo transfer tools, such as CRISPR/Cas9 technology and non-surgical embryo transfer …

Mid-gestation stage mouse embryos were cultured utilizing a serum-free culture medium
prepared from commercially available stem cell media supplements in an oxygenated rolling …