Several culture medium formulations are now available for the successful production and
propagation of viable human embryos. In the most popular format, nutrients are provided in …

The optimal concentrations of individual amino acids, antioxidants, vitamins, osmolytes and
energy sources were determined using a 1-cell Swiss outbred (SO) and or F1 [(CBA× …

STUDY QUESTION What is the relationship between cleavage stage embryo kinetics,
blastocyst metabolism and subsequent embryo viability? SUMMARY ANSWER Embryos …

Several media, some augmented with amino acids, have been formulated recently, based
on simplex optimization, to support the preimplantation development of mouse embryos. For …

In vitro development of rabbit embryos was investigated. Zygotes recovered from female
oviducts at the pronuclear stage (19-22 hpc) were cultured in vitro at standard temperature …

Research to improve mammalian embryo culture media has taken off in recent years
following the development of medium CZB by Chatot et al. in 1989 (1). Three research …

The introduction of stress to embryonic blastomeres through inappropriate culture conditions
results ultimately in the loss of viability. Retention of normal metabolic function in human …

In a review written in 1987 entitled “Pioneering mammalian embryo culture,” John Biggers
(The mammalian preimplantation embryo: regulation of growth and differentiation in vitro …

The field of modern embryo culture spans several decades. However, in the last decade of
the 20th century a resurgence of interest in embryo physiology and metabolism led to highly …