Culture of viable mammalian embryos in vitro

DK Gardner, M Lane - Principles of Cloning, 2014 - Elsevier
The field of modern embryo culture spans several decades. However, in the last decade of
the 20th century a resurgence of interest in embryo physiology and metabolism led to highly …

Humidity level during culture does not affect mouse embryo development

BS Glazar, JA Garcia, PD Cowan, EG Strait… - Fertility and …, 2019 - fertstert.org
Background Embryo culture is routinely performed with or without added humidification to
the incubation chamber. For example, time-lapse incubators typically lack added …

A novel embryo culture media supplement that improves pregnancy rates in mice

AR Highet, T Bianco-Miotto, KG Pringle… - …, 2017 - rep.bioscientifica.com
A novel embryo culture media supplement that improves pregnancy rates in mice Page 1
REPRODUCTION © 2017 Society for Reproduction and Fertility DOI: 10.1530/REP-16-0517 …

Fertilization and early embryology: development of in-vitro-fertilized primate embryos into blastocysts in a chemically defined, protein-free culture medium

RD Schramm, M Al-Sharhan - Human Reproduction, 1996 - academic.oup.com
The formulation of chemically defined culture media that support primate embryo
development would facilitate studies on primate preimplantation embryogenesis. The …

In vitro culture of individual mouse preimplantation embryos: the role of embryo density, microwells, oxygen, timing and conditioned media

RL Kelley, DK Gardner - Reproductive BioMedicine Online, 2017 - Elsevier
Single embryo culture is suboptimal compared with group culture, but necessary for embryo
monitoring, and culture systems should be improved for single embryos. Pronucleate mouse …

Development of in-vitro-fertilized primate embryos into blastocysts in a chemically defined, protein-free culture medium.

RD Schramm, BD Bavister - Human Reproduction (Oxford, England …, 1996 - europepmc.org
The formulation of chemically defined culture media that support primate embryo
development would facilitate studies on primate preimplantation embryogenesis. The …

Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study

WSA Wun, CCC Wun, GM Grunert - Journal of assisted reproduction and …, 1994 - Springer
Purpose Our purpose was to find a medium to enhance mouse zygote development and,
hopefully, to apply the results to a coculture system and to enhance the ART pregnancy rate …

Mammalian embryo culture in a microfluidic device

EM Walters, SG Clark, DJ Beebe… - Germ Cell Protocols …, 2004 - Springer
Over the last decade, the use of in vitro production of mammalian embryos and the utilization
of assisted reproductive technologies (ART), such as non-surgical embryo transfer …

Development of mice and hamster embryos in Ksomaa and HECM-6 medium

B Rosadi, MA Setiadi, D Sajuthi, A Boediono - 2008 - agris.fao.org
< span style=" color:# 29363d; font-family: Tahoma, Arial, Helvetica, sans-serif; font-size:
14px; font-style: italic; text-align: justify; background-color:# ddeaf4;"> The purpose of the …

Preparation of rat serum suitable for mammalian whole embryo culture

M Takahashi, S Makino, T Kikkawa, N Osumi - JoVE (Journal of Visualized …, 2014 - jove.com
Mammalian whole embryo culture (WEC) is a widely used technique for examining
pharmacological toxicity in developing mouse and rat embryos and for investigating the …